Cellular therapy for ocular degeneration

• US 20060280729A1
• Filed: 06/02/2006
• Published: 12/14/2006
• Est. Priority Date: 06/08/2005
• Status: Active Grant

• Current Assignee: Janssen Biotech, Inc. (Johnson & Johnson)
• Original Assignee: Janssen Biotech, Inc. (Johnson & Johnson)
• Inventors: Mistry, Sanjay
• Granted Patent: US 9,074,189 B2
• Time in Patent Office: Days
• US Class Current: 424/93.210
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 9/0048 Eye, e.g. artificial tears
• CPC Class Codes: A61P 27/02 Ophthalmic agents
• CPC Class Codes: A61P 27/06 Antiglaucoma agents or miotics
• CPC Class Codes: A61P 27/12 for cataracts
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: C12N 5/0663 Bone marrow mesenchymal stem cells (BM-MSC)
• CPC Class Codes: C12N 5/0668 Mesenchymal stem cells from other natural sources

Abstract

Compositions and methods applicable to cell-based or regenerative therapy for ophthalmic diseases and disorders comprising mesenchymal stem cells, particularly those characterized by the expression of at least one of the following surface markers: CD29, CD44, CD105 or CD166, and the lack of expression of at least one of CD14, CD34 or CD45.

Claims

1. A method of treating a patient having an ocular degenerative condition, the method comprising administering to the patient mesenchymal stem cells in an amount effective to treat the ocular degenerative condition, wherein the mesenchymal stem cells are characterized by the expression of at least one of the following surface markers:

CD29, CD44, CD105 or CD166, and the lack of expression of at least one of CD14, CD34 or CD45.

2. The method of claim 1, wherein the mesenchymal stem cells promote the survival of ocular cells in situ but the cells do not form structures similar to the photoreceptor layer and do not express a photoreceptor-specific marker.

3. The method of claim 1, wherein the mesenchymal stem cells promote the formation of photoreceptors from ocular cells in situ but the stem cells do not differentiate into photoreceptors themselves.

4. The method of claim 1, wherein the ocular degenerative condition is an acute ocular degenerative condition.

5. The method of claim 4, wherein the acute ocular degenerative condition is a brain trauma, optic nerve trauma or ocular lesion.

6. The method of claim 1, wherein the ocular degenerative condition is a chronic or progressive degenerative condition.

7. The method of claim 6, wherein the chronic or progressive ocular degenerative condition is macular degeneration, retinitis pigmentosa, diabetic retinopathy, glaucoma, limbal epithelial cell deficiency.

8. The method of claim 1, wherein the cells are administered with at least one other cell type.

9. The method of claim 8, wherein the at least one other cell type is an astrocyte, oligodendrocyte, neuron, neural progenitor, neural stem cell, retinal epithelial stem cell, corneal epithelial stem cell, or other multipotent or pluripotent stem cell.

10. The method of claim 8, wherein the at least one other cell type is administered simultaneously with, or before, or after, the mesenchymal stem cells.

11. The method of claim 1, wherein the cells are administered with at least one other agent.

12. The method of claim 11, wherein the at least one other agent is administered simultaneously with, or before, or after, the cells.

13. The method of claim 1, wherein the cells are administered to the surface of an eye.

14. The method of claim 1, wherein the cells are administered to the interior of an eye.

15. The method of claim 14, wherein the cells are administered through a cannula or from a device implanted in the patient'"'"'s body within or in proximity to the eye.

16. The method of claim 14, wherein the cells are administered by implantation of a three dimentional matrix or support containing the cells.

17. A pharmaceutical composition for treating a patient having an ocular degenerative condition, the composition comprising mesenchymal stem cells in an amount effective to treat the ocular degenerative condition, and a pharmaceutically acceptable carrier.

18. The pharmaceutical composition of claim 17, wherein the mesenchymal stem cells are characterized by the expression of at least one of the following surface markers:

CD29, CD44, CD105 or CD166, and the lack of expression of at least one of CD14, CD34 or CD45.

19. The pharmaceutical composition of claim 17 or 18, wherein the ocular degenerative condition is an acute ocular degenerative condition.

20. The pharmaceutical composition of claim 19, wherein the acute ocular degenerative condition is a brain trauma, optic nerve trauma or ocular lesion.

21. The pharmaceutical composition of claim 17 or 18, wherein the ocular degenerative condition is a chronic or progressive degenerative condition.

22. The pharmaceutical composition of claim 21, wherein the chronic or progressive ocular degenerative condition is macular degeneration, retinitis pigmentosa, diabetic retinopathy, glaucoma, limbal epithelial cell deficiency.

23. The pharmaceutical composition of claim 17 or 18, wherein the mesenchymal stem cells promote the survival of ocular cells in situ but the cells do not form structures similar to the photoreceptor layer and do not express a photoreceptor-specific marker.

24. The pharmaceutical composition of claim 17 or 18, wherein the mesenchymal stem cells promote the formation of photoreceptors from ocular cells in situ but the stem cells do not differentiate into photoreceptors themselves.

25. The pharmaceutical composition of claim 17 or 18, containing at least one other cell type.

26. The pharmaceutical composition of claim 25, wherein the at least one other cell type is an astrocyte, oligodendrocyte, neuron, neural progenitor, neural stem cell, retinal epithelial stem cell, corneal epithelial stem cell, or other multipotent or pluripotent stem cell.

27. The pharmaceutical composition of claim 25, comprising at least one other agent.

28. The pharmaceutical composition of claim 27, wherein the at least one other agent is a drug for treating the ocular degenerative disorder.

29. The pharmaceutical composition of claim 17 or 18, wherein the cells are administered to the surface of an eye.

30. The pharmaceutical composition of claim 17 or 18, wherein the cells are administered to the interior of an eye.

31. The pharmaceutical composition of claim 17 or 18, wherein the pharmaceutical carrier is formulated as a three dimentional matrix or support containing the cells.