Label-free methods for performing assays using a colorimetric resonant reflectance optical biosensor
First Claim
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1. A method of detecting binding of molecules released from cells grown in a semi-permeable internal sleeve held in contact with or in close proximity to a colorimetric resonant reflectance optical biosensor comprising:
- a. detecting a colorimetric resonant reflectance optical PWV of a distinct location of the biosensor;
b. growing cells in the semi-permeable internal sleeve held in contact with or in close proximity to the colorimetric resonant reflectance optical biosensor at the distinct location;
c. detecting the colorimetric resonant reflectance optical PWV of the distinct location;
d. comparing the PWV of step (a) to the PWV of step (c);
wherein binding of the molecules released from cells grown in the semi-permeable internal sleeve held in contact with or in close proximity to the colorimetric resonant reflectance optical biosensor to the one or more specific binding substances is detected.
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Abstract
Methods are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also relates to optical devices.
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13 Claims
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1. A method of detecting binding of molecules released from cells grown in a semi-permeable internal sleeve held in contact with or in close proximity to a colorimetric resonant reflectance optical biosensor comprising:
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a. detecting a colorimetric resonant reflectance optical PWV of a distinct location of the biosensor;
b. growing cells in the semi-permeable internal sleeve held in contact with or in close proximity to the colorimetric resonant reflectance optical biosensor at the distinct location;
c. detecting the colorimetric resonant reflectance optical PWV of the distinct location;
d. comparing the PWV of step (a) to the PWV of step (c);
wherein binding of the molecules released from cells grown in the semi-permeable internal sleeve held in contact with or in close proximity to the colorimetric resonant reflectance optical biosensor to the one or more specific binding substances is detected. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification