Biobriefcase

• US 20060281101A1
• Filed: 06/15/2005
• Published: 12/14/2006
• Est. Priority Date: 02/17/2005
• Status: Abandoned Application

• Current Assignee: Lawrence Livermore National Security LLC (Government of the United States of America)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Visuri, Steven R., Mariella, Raymond P. Jr., Dzenitis, John M., Benett, William J., Venkateswaran, Kodumudi S.
• Application Number:
  US11/154,975
• Publication Number:
  US 20060281101A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 1/52 Transportable laboratories; Field kits
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 1/28 Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Qmounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 ; laboratory gas handling apparatus B01L5/00
• CPC Class Codes: G01N 2001/027 field kits / quick test kits
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 35/00 Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Abstract

A bio-briefcase system for analyzing a sample for the presence of biological agents. The bio-briefcase system comprises a housing, an immunoassay section operatively connected to the housing, and/or a nucleic acid assay section operatively connected to the housing.

Claims

1. A bio-briefcase apparatus for analyzing a sample for the presence of biological agents, comprising:

a housing,

an immunoassay section operatively connected to said housing, or

a nucleic acid assay section operatively connected to said housing.

2. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said immunoassay section includes an antibody coupling section.

3. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said immunoassay section includes an antibody coupling section, an antibody reagent section, and microfluidics for moving and mixing the sample.

4. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said immunoassay section includes a photoactivate section.

5. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said immunoassay section includes a photoactivate section with exposure of the sample to light.

6. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said immunoassay section includes a scavenge bound eTags section.

7. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said immunoassay section includes a scavenge bound eTags section, a reagent section, and microfluidics for moving and mixing the sample to scavenge eTags.

8. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said immunoassay section includes a capillary electro-phoresis section.

9. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said nucleic acid assay section includes a lysis section for lysing the sample.

10. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said nucleic acid assay section includes an acoustics lysis section for lysing the sample.

11. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said nucleic acid assay section includes a mechanical lysis section for lysing the sample.

12. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said nucleic acid assay section includes a DNA-capture pillar chip section.

13. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said nucleic acid assay section includes a DNA-capture pillar chip section that concentrates and filters DNA from the sample.

14. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said nucleic acid assay section includes a PCR section to perform PCR amplification.

15. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said nucleic acid assay section includes a flow-through thermal chamber PCR section for performing PCR amplification.

16. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said nucleic acid assay section includes an avidin section for mixing the sample with Avidin to scavenge eTags.

17. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 1 wherein said nucleic acid assay section includes a capillary electro-phoresis section.

18. A bio-briefcase apparatus for analyzing a sample for the presence of biological agents, comprising:

a housing,

an immunoassay means operatively connected to said housing for determining the presence of biological agents, and

a nucleic acid assay means operatively connected to said housing for determining the presence of biological agents.

19. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 18 wherein said immunoassay means includes an antibody coupling section, photoactivate section that exposes the sample to light, an antibody reagent section, a scavenge bound eTags section for moving and mixing the sample to scavenge eTags, a capillary electro-phoresis section, and microfluidics for moving and mixing the sample.

20. The bio-briefcase apparatus for analyzing a sample for the presence of biological agents of claim 18 wherein said nucleic acid assay means includes a lysis section for lysing the sample, a DNA-capture pillar chip section that concentrates and filters DNA from the sample, a PCR section to perform PCR amplification, an avidin section for mixing the sample with Avidin to scavenge eTags, a capillary electro-phoresis section, and microfluidics for moving and mixing the sample.