Enhanced production of lipids containing polyenoic fatty acid by very high density cultures of eukaryotic microbes in fermentors
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Abstract
The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.
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Citations
161 Claims
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1-84. -84. (canceled)
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85. A process for producing lipids containing polyenoic fatty acids from microorganisms of the order Thraustochytriales capable of producing at least about 15% of the total lipids produced by the microorganisms as polyunsaturated lipids comprising conducting a fermentation of the microorganisms in a medium comprising a non-alcohol carbon source, and a limiting nutrient source:
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wherein non-alcohol carbon source and limiting nutrient source are added to the medium to increase the biomass density of the fermentation medium when the dissolved oxygen content of the fermentation medium is at least about 4% of saturation; and
wherein the fermentation of the medium produces lipids containing polyenoic fatty acids when the dissolved oxygen content of the fermentation medium is less than about 3% of saturation. - View Dependent Claims (86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122)
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123. A process for producing lipids containing polyenoic fatty acids from microorganisms of the order Thraustochytriales capable of producing at least about 20% of their biomass as lipids comprising conducting a fermentation of the microorganisms in a medium comprising a source of carbon:
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wherein the source of carbon is added to the fermentation medium in a fed-batch process to increase the biomass density of the fermentation medium when the dissolved oxygen content of the fermentation medium is at least about 4% of saturation; and
wherein the fermentation of the medium produces lipids containing polyenoic fatty acids when the dissolved oxygen content of the fermentation medium is less than about 3% of saturation. - View Dependent Claims (124, 125, 126, 127)
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128. A process for producing lipids containing polyenoic fatty acids from microorganisms of the order Thraustochytriales capable of producing at least about 20% of their biomass as lipids comprising conducting a fermentation of the microorganisms in a medium comprising a non-alcohol carbon source, and a limiting nutrient source:
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wherein non-alcohol carbon source and limiting nutrient source are added to the medium to increase the biomass density of the fermentation medium when the dissolved oxygen content of the fermentation medium is at least about 4% of saturation; and
wherein the fermentation of the medium produces lipids containing polyenoic fatty acids when the dissolved oxygen content of the fermentation medium is less than about 3% of saturation. - View Dependent Claims (129, 130, 131, 132, 133)
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134. A process for producing lipids containing polyenoic fatty acids from microorganisms of the order Thraustochytriales capable of producing at least about 15% of the total lipids produced by the microorganisms as polyunsaturated lipids comprising conducting a fermentation of the microorganisms in a medium comprising a source of carbon:
- wherein the source of carbon is added to the fermentation medium in a fed-batch process to increase the biomass density of the fermentation medium when the dissolved oxygen content of the fermentation medium is at least about 4% of saturation; and
wherein the fermentation of the medium produces lipids containing polyenoic fatty acids when the dissolved oxygen content of the fermentation medium is less than about 3% of saturation. - View Dependent Claims (135, 136, 137, 138)
- wherein the source of carbon is added to the fermentation medium in a fed-batch process to increase the biomass density of the fermentation medium when the dissolved oxygen content of the fermentation medium is at least about 4% of saturation; and
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139. A process for growing microorganisms of the order Thraustochytriales capable of producing at least about 20% of their biomass as lipids comprising conducting a fermentation of the microorganisms in a medium comprising a carbon source, and a limiting nutrient source:
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wherein carbon source and limiting nutrient source are added to the medium to increase the biomass density of the fermentation medium when the dissolved oxygen content of the fermentation medium is at least about 4% of saturation;
wherein the fermentation of the medium produces lipids containing polyenoic fatty acids when the dissolved oxygen content of the fermentation medium is less than about 3% of saturation;
wherein the process produces at least an average rate of about 0.5 g/L/hr of lipids; and
wherein at least about 15% of the total lipids produced by the microorganisms is polyunsaturated lipids. - View Dependent Claims (140, 141, 142, 143)
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144. A process for growing microorganisms of the order Thraustochytriales comprising conducting a fermentation of the microorganisms in a medium comprising a carbon source, and a limiting nutrient source:
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wherein the carbon source and limiting nutrient source are added to the medium to increase the biomass density of the fermentation medium when the dissolved oxygen content of the fermentation medium is at least about 4% of saturation;
wherein the fermentation of the medium produces lipids containing polyenoic fatty acids when the dissolved oxygen content of the fermentation medium is less than about 3% of saturation; and
wherein the microorganisms are capable of producing at least about 20% of their biomass as lipids comprising some polyenoic fatty acids. - View Dependent Claims (145, 146, 147, 148)
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150. A process for producing microbial lipids comprising:
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growing microorganisms of the order Thraustochytriales in a fermentation medium to increase the biomass density of the fermentation medium;
allowing the microorganisms to produce the lipids when the dissolved oxygen content of the fermentation medium is less than about 3% of saturation; and
recovering the lipids, wherein greater than about 15% of the lipids are polyunsaturated lipids. - View Dependent Claims (151, 152, 153, 154, 155)
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156. A process for producing microbial lipids comprising conducting a fermentation of microorganisms in a medium comprising a carbon source, and a limiting nutrient source:
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adding carbon source and limiting nutrient source to a fermentation medium when the level of dissolved oxygen in the fermentation medium is at least about 4% of saturation;
producing lipids when the level of dissolved oxygen in the fermentation medium is about 3% of saturation or less; and
recovering the microbial lipids;
wherein at least about 15% of the microbial lipids are polyunsaturated lipids. - View Dependent Claims (157, 158, 159, 160, 161)
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Specification