Method for producing a colorimetric resonant reflection biosensor on rigid surfaces

• US 20060286663A1
• Filed: 07/13/2006
• Published: 12/21/2006
• Est. Priority Date: 10/30/2000
• Status: Active Grant

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Cunningham, Brian T., Qiu, Jean
• Granted Patent: US 7,615,339 B2
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus

Abstract

The invention provides methods for fabricating calorimetric resonant reflection biosensors.

Claims

1. A method for casting a colorimetric resonant reflection biosensor structure comprising:

(a) applying a liquid or semi-solid material that is capable of being transformed or cured into a flexible master structure over a rigid colorimetric resonant reflection biosensor master structure;

(b) transforming or curing the liquid or semi-solid material into a flexible master structure, wherein the flexible master structure has the rigid calorimetric resonant reflection biosensor master structure embossed into a first surface of the flexible master structure; and

(c) peeling the flexible master structure from the rigid calorimetric resonant reflection biosensor master structure;

(d) pressing the flexible master structure into a liquid or semi-solid layer, wherein the liquid or semi-solid layer is on a rigid substrate;

whereby a colorimetric resonant reflection biosensor structure is casted.

2. The method of claim 1., wherein the rigid calorimetric resonant reflection biosensor master structure is etched into a silicon or glass wafer surface.

3. The method of claim 1, wherein the flexible master structure comprises a grating pattern selected from the group consisting of squares, triangles, sinusoidal waves, inverted “

u”

shapes, lines, circles, ellipses, trapezoids, ovals, rectangles, hexagons, phase-quantized terraced surface relief patterns whose groove pattern resembles a stepped pyramid, and concentric rings.

4. The method of claim 1, wherein the flexible master structure comprises a grating pattern having a periodic spacing of between about 0.1 microns to about 2.0 microns.

5. The method of claim 1, wherein the flexible master structure comprises a submicron grating pattern.

6. The method of claim 5, wherein the grating pattern has a periodic spacing of between about 0.2 microns to about 0.6 microns.