Methods and apparatuses for culturing stem cell using biomaterial shell

• US 20060286666A1
• Filed: 06/17/2005
• Published: 12/21/2006
• Est. Priority Date: 06/17/2005
• Status: Abandoned Application

• Current Assignee: Korea Advanced Institute of Science and Technology
• Original Assignee: Korea Advanced Institute of Science and Technology
• Inventors: Na, Dae Suk, Chae, Su Kyoung, Kim, Choong, Lee, Jin Woo, Ju, Byeong Kwon, Kang, Ji Yoon
• Application Number:
  US11/155,532
• Publication Number:
  US 20060286666A1
• Time in Patent Office: Days
• US Class Current: 435/325
• CPC Class Codes: C12N 2533/10 Mineral substrates
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696

Abstract

Disclosed are methods and apparatuses for culturing stem cell using biomaterial shell, e.g. fertilized zebrafish chorions, which may induce formation of embryonic bodies, cell proliferation, and cell differentiation to other types effectively inside the chorions without adding any inducing agents or cell differentiation stimulants. There is provided an apparatus for culturing stem cell using biomaterial shell comprising: a microwell layer having a plurality of microwells; a microhole layer having a plurality of microholes, the microhole layer being located under the microwell layer, diameter of the microholes being smaller than diameter of the microwells and diameter of the biomaterial shell; and a flow-channel layer having a plurality of flow-channels for supplying cell medium to the microholes, the flow-channel layer being located under the microhole layer, and wherein the stem cell is encapsulated by the biomaterial shell in the microwell.

Claims

1-9. -9. (canceled)

10. A method for culturing stem cell using biomaterial shell, comprising:

injecting the stem cell into the biomaterial shell located in microwell; and

loading the apparatus comprising the microwell into CO₂ incubator which contains 10% fetal bovine serum (FBS) to culture the stem cell encapsulated by the biomaterial shell.

11. The method of claim 10, further comprising:

supplying cell medium to the biomaterial shell by inputting the cell medium through inlet/outlet connected through flow-channel to the microwell.

12. The method of claim 10, wherein the biomaterial shell has nano pore surface structure to allow cell medium to flow into the biomaterial shell.

13. The method of claim 10, wherein said injecting the stem cell into the biomaterial shell comprises:

making an opening on the biomaterial shell; and

injecting a jet of distilled water and ethanol through the opening on the biomaterial shell.

14. The method of claim 10, wherein the stem cell is P19 embryonic carcinoma (P19EC).

15. The method of claim 10, wherein the biomaterial shell is fertilized chorion.

16. The method of claim 15, wherein the inner material of the fertilized chorion is removed by injecting a jet of distilled water and ethanol through opening on the fertilized chorion.

17-22. -22. (canceled)