Crystallization of IGF-1

• US 20060287235A1
• Filed: 06/20/2006
• Published: 12/21/2006
• Est. Priority Date: 02/09/2001
• Status: Active Grant

• Current Assignee: Felix Vajdos, Mark Ultsch, Michelle Schaffer
• Original Assignee: Felix Vajdos, Mark Ultsch, Michelle Schaffer
• Inventors: Ultsch, Mark, Schaffer, Michelle, Vajdos, Felix
• Granted Patent: US 7,297,763 B2
• Time in Patent Office: Days
• US Class Current: 530/324
• CPC Class Codes: A61P 13/12 of the kidneys
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 3/04 Anorexiants; Antiobesity agents
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 31/18 for HIV
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07K 14/65 Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
• CPC Class Codes: C07K 2299/00 Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Abstract

Crystalline IGF-1 is provided along with a method for production thereof. Crystallizing IGF-1 comprises the steps of mixing an aqueous solution comprising IGF-1 with a reservoir solution comprising a precipitant to form a mixture; and crystallizing the mixture, optionally also recrystallizing and isolating the crystalline IGF-1. In addition, a method for identifying IGF-1 indirect agonists is provided using a detergent as a standard for the level of inhibition of binding of IGFBP-1 or IGFBP-3 to IGF-1 and/or using the coordinates of the binding pockets of IGF-1 to which a candidate indirect agonist binds for structure-based drug design.

Claims

1. A co-crystalline complex of IGF-1 and N,N-bis(3-D-gluconamidopropyl)-d-eoxycholamine.