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Marker for undifferentiated state of cell and composition and method for separation and preparation of stem cells

  • US 20060288431A1
  • Filed: 02/12/2004
  • Published: 12/21/2006
  • Est. Priority Date: 02/13/2003
  • Status: Abandoned Application
First Claim
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1. A nucleic acid molecule, comprising:

  • (a) a polynucleotide having a base sequence set forth in SEQ ID NO. 1, 3, 5 or 29, or a fragment thereof;

    (b) a polynucleotide encoding a polypeptide consisting of an amino acid sequence set forth in SEQ ID NO. 2, 4, 6 or 30, or a fragment thereof;

    (c) a polynucleotide encoding a variant polypeptide having an amino acid sequence set forth in SEQ ID NO. 2, 4, 6 or 30, or a fragment thereof, wherein at least one amino acid in the sequence has a mutation selected from the group consisting of substitution, addition, and deletion and wherein the variant polypeptide has biological activity;

    (d) a polynucleotide, which is a spliced mutant or alleic mutant of a base sequence set forth in SEQ ID NO. 1, 3, 5 or 29, or a fragment thereof;

    (e) a polynucleotide encoding a species homolog of a polypeptide consisting of an amino acid sequence set forth in SEQ ID NO. 2, 4, 6 or 30, or a fragment thereof;

    (f) a polynucleotide hybridizable to any one of the polynucleotides of (a) to (e) under stringent conditions and encoding a polypeptide having biological activity;

    or (g) a polynucleotide consisting of a base sequence having at least 70% identity to any one of the polynucleotides (a) to (e) or a complementary sequence thereof, and encoding a polypeptide having biological activity.

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