Reactive oxygen species measuring device

• US 20060289313A1
• Filed: 02/24/2004
• Published: 12/28/2006
• Est. Priority Date: 02/24/2003
• Status: Active Grant

• Current Assignee: Fumio Mizoguchi, Hitoshi Takebayashi, Makoto Yuasa, Masahiko Abe, Masahiko Yokosuka, Masamitsu Nagahama, Masayuki Kawasaki, Masumi Koishi, Rizwangul Ibrahim, Shigeru Kido, Takashi Ishihara
• Original Assignee: Fumio Mizoguchi, Hitoshi Takebayashi, Makoto Yuasa, Masahiko Abe, Masahiko Yokosuka, Masamitsu Nagahama, Masayuki Kawasaki, Masumi Koishi, Rizwangul Ibrahim, Shigeru Kido, Takashi Ishihara
• Inventors: Kawasaki, Masayuki, Yokosuka, Masahiko, Ishihara, Takashi, Yuasa, Makoto, Kido, Shigeru, Mizoguchi, Fumio, Koishi, Masumi, Nagahama, Masamitsu, Ibrahim, Rizwangul, Takebayashi, Hitoshi, Abe, Masahiko
• Granted Patent: US 8,298,387 B2
• Time in Patent Office: Days
• US Class Current: 205/782
• CPC Class Codes: A61B 5/14542 for measuring blood gases A61B5/14551 takes precedence
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 27/404 Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid , e.g. Clark-type oxygen sensors

Abstract

A reactive oxygen species measuring device according to the present invention comprises a reactive oxygen species sensor provided with an electrode assembly capable of detecting the presence of reactive oxygen species or the like in terms of an electric current, a power source means for applying a measuring voltage to the reactive oxygen species sensor, and a reactive oxygen species concentration measuring means for measuring a concentration of the reactive oxygen species or the like from the current detected by the reactive oxygen species sensor. According to the present invention, a concentration of reactive oxygen species or the like such as in vivo or in vitro superoxide anion radical (O₂⁻.) can be measured reliably, and the entire device can be formed in a smaller size and always mounted to a living body. In addition, the measured data can be transmitted to a remote place or location, and further, the health condition of a living body or the like determined in the remote place can be returned to a measuring place and informed, and thus, the health condition of the living body can be monitored by measuring and monitoring reactive oxygen species.

Claims

1. A reactive oxygen species measuring device comprising a reactive oxygen species sensor provided with an electrode assembly capable of detecting the presence of reactive oxygen species or the like in terms of an electric current, a power source means for applying a measuring voltage to said reactive oxygen species sensor, and a reactive oxygen species concentration measuring means for measuring a concentration of the reactive oxygen species or the like from said current detected by said reactive oxygen species sensor.

2. A reactive oxygen species measuring device according to claim 1, characterized in that said reactive oxygen species concentration measuring means includes an external output means for delivering the measured concentration of the reactive oxygen species or the like to the outside, and further includes a remotely monitoring means adapted to monitor the concentration of the active oxygen species or the like under the reception of the output from said external output means.

3. An active oxygen species measuring device according to claim 2, characterized in that said remotely monitoring means includes a communication means for transmitting and receiving data to and from said reactive oxygen species measuring device, an arithmetic means for conducting a data processing for the concentration of the reactive oxygen species or the like received from said external output means, and a display means for display the concentration of the reactive oxygen species or the like and the result of the data processing provided by said arithmetic means, and

said external output mans in said reactive oxygen species measuring device is comprised of a communication means for transmitting and receiving the data to and from the remotely monitoring means, and a display means for displaying the concentration of the reactive oxygen species or the like and the data received from said remotely monitoring means.

4. A remotely controlled reactive oxygen species measuring system according to claim 2 or 3, characterized in that the delivery and reception of the data between said reactive oxygen species measuring device and said remotely monitoring means are carried out through a radio or wire propagation system.

5. A remotely controlled reactive oxygen species measuring system according to claim 2 or 3, characterized in that the communication means and the display means of said reactive oxygen species measuring device are formed in a mobile phone to deliver and receive the data to and from said remotely monitoring means through an internet.

6. A reactive oxygen species measuring device according to any of claims 1 to 5, characterized in that the electrode of said reactive oxygen species sensor is formed into a shape capable of detecting the presence of an in vivo reactive oxygen species or the like in terms of an electric current.

7. A reactive oxygen species measuring device according to any of claims 1 to 6, characterized in that said power source means is formed so that the measuring voltage to be applied to said reactive oxygen species sensor can be controlled variably.

8. A reactive oxygen species measuring device according to any of claims 1 to 7, characterized in that said reactive oxygen species concentration measuring means includes at least one of a display means for displaying a concentration of reactive oxygen species or the like determined based on the electric current detected by said reactive oxygen species sensor, and an external output means for delivering data to the outside.

9. A reactive oxygen species measuring device according to any of claims 1 to 8, characterized in that the electrode of said reactive oxygen species sensor includes, on its surface, an element capable of detecting the presence of active oxygen species or the like in terms of an electric current.

10. A reactive oxygen species measuring device according to claim 9, characterized in that said element capable of detecting the presence of reactive oxygen species or the like in terms of an electric current comprises at least one of a polymerized film of a metal porphyrin complex, a redox polymer or its derivative, a metal composite and a complex-based compound.

11. An active oxygen species measuring device according to claim 10, characterized in that said metalloporphyrin complex is represented by the following formula (1) or (II):

(I)

wherein M indicates a metal ion selected from the group consisting of iron, manganese, a cobalt, chromium and iridium;

at least one of four Rs is any one of thiofuryl, pyrrolyl, furyl, mercaptophenyl, aminophenyl and hydroxyphenyl groups; and

each of other Rs indicates any of the above-described groups, or alkyl group, aryl group or hydrogen; and

(II)

wherein each of M and R has the above-described meaning, and at least one of two Ls is an nitrogen-based axial ligand such as imidazole and its derivative, pyridine and its derivative, aniline and its derivative, histidine and its derivative, and trimethylamine and its derivative, a sulfur-based axial ligand such as thiophenol and its derivative, cysteine or its derivative, and methionine and its derivative, or an oxygen-based axial ligand such as benzoic acid and its derivative, acetic acid and its derivative, phenol and its derivative, an aliphatic alcohol and its derivative or water; and

the other L indicates any of the above-described axial ligands or no ligand.

12. A reactive oxygen species measuring device according to claim 10 or 11, characterized in that the porphyrin compound forming said metal porphyrin complex is one selected from the group consisting of 5,10,15,20-tetrakis(2-thienyl)porphyrin, 5,10,15,20-tetrakis(3-thienyl)porphyrin, 5,10,15,20-tetrakis(2-pyrrolyl)porphyrin, 5,10,15,20-tetrakis(3-pyrrolyl)porphyrin, 5,10,15,20-tetrakis(2-furyl)porphyrin, 5,10,15,20-tetrakis(3-furyl)porphyrin, 5,10,15,20-tetrakis(2-mercaptophenyl)porphyrin, 5,10,15,20-tetrakis(3-mercaptophenyl)porphyrin, 5,10,15,20-tetrakis(4-mercaptophenyl)porphyrin, 5,10,15,20-tetrakis(2-aminophenyl)porphyrin, 5,10,15,20-tetrakis(3-aminophenyl)porphyrin, 5,10,15,20-tetrakis(4-aminophenyl)porphyrin, 5,10,15,20-tetrakis(2-hydroxyphenyl)porphyrin, 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin, 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin, [5,10,15-tris(2-thienyl)-20-mono(phenyl)]porphyrin, [5,10,15-tris(3-thienyl)-20-mono(phenyl)]porphyrin, [5,10-bis(2-thienyl)-15,20-di(phenyl)]porphyrin, [5,10-bis(3-thienyl)-15,20-di(phenyl)]porphyrin, [5,10-bis(2-thienyl)-10,20-di(phenyl)]porphyrin, [5,10-bis(3-thienyl)-10,20-di(phenyl)]porphyrin, [5-mono(2-thienyl)-10,15,20-tri(phenyl)]porphyrin, [5-mono(3-thienyl)-10,15,20-tri(phenyl)]porphyrin.