Methods of identifying genomic and proteomic biomarkers for cystic fibrosis, arrays comprising the biomarkers and methods of using the arrays
First Claim
Patent Images
1. A method of determining the level of expression of a population of proteins in a first cell comprising:
- growing the first cells in a medium comprising radio-labeled amino acids such that the radio-labeled amino acid is incorporated into the proteins;
lysing the first cells;
placing the first cell lysate on a first gel;
separating the proteins in the first cell lysate using 2-D gel electrophoresis; and
imaging the first gel using autoradiography.
5 Assignments
0 Petitions
Accused Products
Abstract
Cystic fibrosis (CF) is the most common fatal autosomal recessive disease in the U.S. and is principally caused by the DF508 mutation the CFTR gene. The principal site of morbidity and mortality for this disease is the lung. We have used genomic and proteomic methods to identify ubiquitin carboxy terminal hydrolase-1 (UCHL1) as a biomarker for cystic fibrosis. Both gene expression and cognate protein expression are massively upregulated in CF lung epithelial cells. We suggest that this gene can be useful in the assembly of a diagnostic or prognostic chip for CF, or as a target for therapeutic intervention.
-
Citations
30 Claims
-
1. A method of determining the level of expression of a population of proteins in a first cell comprising:
-
growing the first cells in a medium comprising radio-labeled amino acids such that the radio-labeled amino acid is incorporated into the proteins;
lysing the first cells;
placing the first cell lysate on a first gel;
separating the proteins in the first cell lysate using 2-D gel electrophoresis; and
imaging the first gel using autoradiography. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
-
-
11. An array comprising:
a plurality of different probes disposed on a surface of a solid support, wherein each of the different probes bind to a different marker for cystic fibrosis. - View Dependent Claims (12, 13, 14, 15, 16, 17)
Specification