Method for the quality control of functionalized surfaces

• US 20060292612A1
• Filed: 06/14/2006
• Published: 12/28/2006
• Est. Priority Date: 06/15/2005
• Status: Abandoned Application

• Current Assignee: Febit Holding GmbH (FEBIT)
• Original Assignee: Febit Biotech GmbH
• Inventors: Scheffler, Matthias
• Application Number:
  US11/452,422
• Publication Number:
  US 20060292612A1
• Time in Patent Office: Days
• US Class Current: 435/6.12
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00574 radioactive
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00578 electrophoric
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00657 One-dimensional arrays
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00693 Means for quality control
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00729 : Peptide nucleic acids [PNA]
• CPC Class Codes: B01J 2219/00731 : Saccharides
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/532 : Production of labelled immunochemicals
• CPC Class Codes: G01N 33/5436 : with ligand physically entrapped within the solid phase liposomes G01N33/5432; immunological test elements G01N33/54386
• CPC Class Codes: G01N 33/58 : involving labelled substances G01N33/53 takes precedence

Abstract

The invention relates to a method which can be used to test firstly a reaction and secondly the distribution of functional groups on a surface. This method is employed in a situation that demands documentation or testing of a distribution of surface functionalization, which is as homogeneous as possible.

Claims

1. A method for testing functionalized groups on a surface,

(a) providing a support comprising a surface,

(b) coating at least part of the surface of said support with first functional coating molecules, using first coating molecules which carry a reversibly bound label,

(c) detecting the existence and/or density or/and distribution of the labels on the surface,

(d) removing the labels and

(e) where appropriate, coating the support with further functional coating molecules, which react with functional groups of said first coating molecules, said functional groups having preferably been uncovered due to removal of the labeling groups.

2. The method as claimed in claim 1 for preparing supports for biochips, gene chips and microarrays.

3. The method as claimed in claim 1, wherein the further functional coating molecules are selected from:

(a) nucleic acids or nucleic acid analogs or building blocks for the synthesis thereof,

(b) peptides or proteins or building blocks for the synthesis thereof,

(c) carbohydrates or building blocks for the synthesis thereof,

(d) compounds of combinatorial chemistry or building blocks for the synthesis thereof, and

(e) linker molecules or building blocks for the synthesis thereof.

4. A reagent for carrying out the method as claimed in claim 1, containing at least one first functional coating molecule having a reversibly bound label and at least one further functional coating molecule capable of reacting with functional groups of said first coating molecule, said functional groups having preferably been uncovered due to removal of the labeling groups.