Biosensor and method of analyte measuring
First Claim
1. A sensor comprising a magnetic sensor, which has a plurality of magnetic field detection elements arranged two dimensionally in X rows and Y columns (X and Y are natural numbers), said detection elements each generating an output value according to the intensity of a detected magnetic field, and measures an amount of magnetic particles on said magnetic sensor based on said output values, wherein said sensor has a signal processing means by which said amount of magnetic particles is determined based on a dispersion of an output value distribution obtained from said output values of the plurality of magnetic field detection elements.
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Abstract
A biosensor capable of analyzing an object, such as antigen, antibody, DNA or RNA, through detection of magnetic field to thereby allow washout of unbound label molecules to be unnecessary, which biosensor is compact and available at low price, excelling in detection precision. Coils are arranged at an upper part and a lower part of a magnetic sensor using a hall element as a magnetic field detection element. An object and magnetic particles having an antibody capable of specific bonding with the object bound to the surface thereof are introduced in the magnetic sensor having a molecular receptor capable of specific bonding with the object attached to the surface thereof. Therefore, a change in magnetic field by magnetic particles bonded through the molecular receptor to the surface of the magnetic sensor is detected by means of the hall element. At that time, one applied magnetic field is set so that the magnetization intensity of magnetic particles falls within the range from initial magnetic permeability to maximum magnetic permeability while another applied magnetic field is set so that the magnetization intensity of some or all of the magnetic particles becomes saturated, and output signals are compared with each other. Thus, the amount of bonded magnetic particles can be identified.
27 Citations
31 Claims
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1. A sensor comprising a magnetic sensor, which has a plurality of magnetic field detection elements arranged two dimensionally in X rows and Y columns (X and Y are natural numbers), said detection elements each generating an output value according to the intensity of a detected magnetic field, and measures an amount of magnetic particles on said magnetic sensor based on said output values,
wherein said sensor has a signal processing means by which said amount of magnetic particles is determined based on a dispersion of an output value distribution obtained from said output values of the plurality of magnetic field detection elements.
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5. A biosensor comprising a magnetic sensor, which has a plurality of magnetic field detection elements arranged two dimensionally in X rows and Y columns (X and Y are natural numbers), said detection elements each generating an output value according to the intensity of a detected magnetic field, and measures an amount of magnetic particles on said magnetic sensor based on said output values,
further comprising: -
a means for applying external magnetic field of greatly different intensities to said magnetic sensor bound to said magnetic particles;
a signal processing means that determines the amount of said bound magnetic particles by comparing output values of respective said magnetic field detection elements when the external magnetic field of greatly different intensities is applied, and wherein one of said external magnetic field of greatly different intensities is such a strong magnetic field that the magnetization intensity of at least some of said bound magnetic particles becomes saturated, and another external magnetic field is such a weak magnetic field that of said bound magnetic particles each have the magnetic permeability falling within a range from an initial magnetic permeability to a maximum magnetic permeability. - View Dependent Claims (6)
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28. A biosensor comprising a magnetic sensor, in which a plurality of magnetic field detection elements are arranged, said elements each producing an output value corresponding to the intensity of the detected magnetic field;
- and a signal processing means that determines an amount of magnetic each particles bound to said magnetic sensor based on respective output values obtained from said plurality of magnetic field detection elements,
wherein said signal processing means judges the dispersion condition of said magnetic particles based on the output values obtained from said plurality of magnetic field detection elements, after introducing said magnetic particles to said magnetic sensor and before removing said unbound magnetic particles from said magnetic sensor.
- and a signal processing means that determines an amount of magnetic each particles bound to said magnetic sensor based on respective output values obtained from said plurality of magnetic field detection elements,
Specification