Spectroscopic analysis of a biological fluid reacted with an enzyme
First Claim
1. A method for measuring the presence of an analyte in a sample, said method comprising:
- reacting the sample with an enzyme that is reactive with the analyte;
measuring a reacting sample spectrum, where said reacting sample spectrum is a infrared absorption spectrum of the sample during said reacting; and
determining the concentration of the analyte in the sample from said reacting sample spectrum.
3 Assignments
0 Petitions
Accused Products
Abstract
An apparatus is presented for estimating the concentration of an analyte using a combined enzyme-spectroscopic method. Examples are provided for the detection of glucose and lactate. A sample of biological fluid is mixed or contacted with an enzyme specific to the analyte of interest, and the reacting fluid is probed with an optical system at wavelengths that includes at least one wavelength that is sensitive to the analyte concentration and at least one wavelength that is not sensitive to the analyte concentration. The optical system measures properties, such as optical density, and relates the measurements to concentration through a calibration of the system. A method is also provided for analyzing the data obtained from optical measurements of reactions of enzymes with biological fluids. These technologies may be applied to continuous or periodic patient sampling systems or to test strip type devices.
18 Citations
30 Claims
-
1. A method for measuring the presence of an analyte in a sample, said method comprising:
-
reacting the sample with an enzyme that is reactive with the analyte;
measuring a reacting sample spectrum, where said reacting sample spectrum is a infrared absorption spectrum of the sample during said reacting; and
determining the concentration of the analyte in the sample from said reacting sample spectrum. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
-
22. An apparatus for accepting a material sample having an initial analyte concentration comprising:
-
an enzyme for reacting with the accepted material sample;
an optical system to measure an optical property of the reacting material sample at at least two wavelengths; and
a processor programmed to determine the initial analyte concentration from the measured optical properties. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30)
-
Specification