Detection of analytes

• US 20070004046A1
• Filed: 07/01/2005
• Published: 01/04/2007
• Est. Priority Date: 07/01/2005
• Status: Abandoned Application

• Current Assignee: Platypus Technologies, LLC
• Original Assignee: Platypus Technologies, LLC
• Inventors: Abbott, Nicholas
• Application Number:
  US11/173,122
• Publication Number:
  US 20070004046A1
• Time in Patent Office: Days
• US Class Current: 436/104
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 2430/10 Insecticides
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10T 436/163333 Organic [e.g., chemical warfare agents, insecticides, etc.]

Abstract

The present invention relates to the field of detection of analytes, and in particular to detection of organophosphates using a liquid crystal assay format and a variety of analytes in stand-off detection formats utilizing liquid crystals as part of reporting system. The present devices find use in detecting cumulative exposure to organophosphates in the aerosol phase.

Claims

1. A method of remotely detecting an analyte comprising:

a) providing a plurality of liquid crystal assay devices comprising a first surface displaying a recognition moiety, said first surface in contact with a liquid crystal;

b) exposing said plurality of liquid crystal assay devices to a sample suspected of containing said analyte; and

c) simultaneously irradiating said plurality of liquid crystal assay devices under conditions such that radiation returned from said plurality of liquid crystal assay devices is indicative of a change in orientation of said liquid crystal in said assay devices caused by interaction of said analyte with said recognition moiety.

2. The method of claim 1, wherein said irradiating is performed by exposure to electromagnetic radiation.

3. The method of claim 1, wherein said electromagnetic radiation is selected from the group consisting of visible light, x-ray radiation, UV radiation, infrared radiation, and radiofrequency radiation.

4. The method of claim 1, wherein said radiation returned from said assay device is measured by a method selected from the group consisting of infrared spectroscopy, raman spectroscopy, x-ray spectroscopy, visible light spectroscopy, ultraviolet spectroscopy, spectroscopy of radiofrequency radiation, and combinations thereof.

5. The method of claim 1, wherein said radiation returned from said device exhibits a peak wavelength that is different in the presence of an analyte than in the absence of an analyte.

6. The method of claim 1, wherein said radiation returned from said device exhibits a spectrum that is different in the presence of an analyte than in the absence of an analyte.

7. The method of claim 1, wherein said radiation returned from said device exhibits a change in the intensity of the peak of the spectrum emitted from said device.

8. The method of claim 1, wherein said liquid crystal comprises mesogens selected from the group consisting of E7, MLC, 5CB (4-n-pentyl-4′

-cyanobiphenyl), and 8CB (4-cyano-4′

octylbiphenyl).

9. The method of claim 1, wherein said liquid crystal assay devices are irradiated by a remote radiation source.

10. The method of claim 9, wherein said remote radiation source is greater than 10 meters from said liquid crystal assay device.

11. A system for remotely detecting an analyte comprising:

a) a plurality of liquid crystal assay devices comprising a first surface displaying a recognition moiety, said first surface in contact with a liquid crystal;

b) a radiation source remote from said plurality of liquid crystal assay devices; and

c) a detector configured to receive a signal from said plurality of assay devices upon radiation of said plurality of assay devices by said radiation source.

12. A method of assaying cumulative exposure to organophosphates comprising:

a) providing a device comprising a liquid crystal, said liquid crystal between a first surface and a second surface, said first surface comprising an organic layer in contact with said first surface, said organic layer having immobilized thereon at least one metal ion, said device having an opening therein; and

b) exposing said device to a sample suspected of containing organophosphates, wherein cumulative exposure to organophosphates is indicated by a change in the orientation of said liquid crystal identified as wavefront advancing from said opening.

13. The method of claim 12, wherein said organic layer has immobilized thereon a plurality of different metal ions selected from the group consisting of Al³⁺, Ag¹⁺, Ba³⁺, Cd²⁺, Ce³⁺, Co²⁺, Cr³⁺, Eu³⁺, Fe²⁺, Fe³⁺, Ga³⁺, In³⁺, Mn²⁺, Ni²⁺, Pb²⁺, Pr³⁺, and Zn²⁺.

14. The method of claim 12, wherein said metal ions are arranged in an array.

15. A method of identifying a particular organophosphate comprising:

a) providing a substrate comprising at least two detection regions having at least two different metal ions immobilized thereon; and

b) exposing said device to a sample suspected of containing an organophosphate; and

c) determining the identity of said organophosphate by examining the change of liquid crystal orientation in said detection regions.

16. The method of claim 15, wherein said liquid crystal overlaying said detection regions is disordered in the presence of organophosphates.

17. The method of claim 15, wherein said detection region has immobilized thereon a plurality of different metal ions selected from the group consisting of Al³⁺, Ag¹⁺, Ba³⁺, Cd²⁺, Ce³⁺, Co²⁺, Cr³⁺, Eu³⁺, Fe²⁺, Fe³⁺, Ga³⁺, In³⁺, Mn²⁺, Ni²⁺, Pb²⁺, Pr³⁺, and Zn²⁺.

18. A device comprising at least a first substrate having a surface, said substrate comprising at least first and second detection regions on said surface, wherein said first and second detection regions comprise an organic layer and a metal ion immobilized on said organic layer and wherein said metal ions on said first and second detection regions are different.

19. A device comprising:

at least a first substrate having a surface, said substrate further comprising at least a first detection region on said surface, wherein said detection region comprises a recognition moiety;

a liquid crystal in contact with said first substrate; and

a housing having an opening therein, said substrate configured in said housing so that said detection region is exposed to the atmosphere through said opening.

20. The device of claim 19, wherein said housing is movable between an exposure position wherein said detection region is exposed to the atmosphere through said opening and a reading position wherein said detection region is substantially closed off to the atmosphere.