Slide deposition chamber

• US 20070009389A1
• Filed: 07/08/2005
• Published: 01/11/2007
• Est. Priority Date: 07/08/2005
• Status: Abandoned Application

• Current Assignee: Ikonisys, Inc.
• Original Assignee: Ikonisys, Inc.
• Inventors: Kilpatrick, Michael, Tafas, Triantafyllos, Seppo, Antti
• Application Number:
  US11/177,036
• Publication Number:
  US 20070009389A1
• Time in Patent Office: Days
• US Class Current: 422/99
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 1/2813 Producing thin layers of samples on a substrate, e.g. smearing, spinning-on G01N1/30 takes precedence
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides

Abstract

A cell deposition chamber and a method for depositing a biological sample on a microscope slide using the chamber. The chamber is removably sealed onto a sample receiving area on the slide and has a sealable opening to allow aspiration of the sample from the slide surface. The chamber is configured to allow the aspiration of all areas of the slide surface by a pipette and the chamber drains fully when inverted. The chamber can be fitted with a disposable liner to make the chamber reusable.

Claims

1. An apparatus comprising:

a chamber defining a void and having a footprint configured to fit over a receiving area on a microscope slide, said chamber having a top portion, a middle portion and a bottom portion, said top portion defining an opening of said chamber to allow a biological sample to be deposited on the surface of said microscope slide;

a means for closing and sealing said opening; and

a means for attaching said chamber to said receiving area on said microscope slide.

2. The apparatus according to claim 1, wherein said chamber has an open bottom with a polygonal footprint.

3. The apparatus according to claim 1, wherein said footprint is circular.

4. The apparatus according to claim 1, wherein said top portion of said chamber comprises a cylindrical shape.

5. The apparatus according to claim 1, wherein said means for closing and sealing said opening is a cap.

6. The apparatus according to claim 1, wherein said means for closing and sealing said opening is a plug.

7. The apparatus according to claim 1, wherein said middle portion of said chamber comprises a truncated polyhedron to allow said biological sample to be aspirated from all areas of said slide surface.

8. The apparatus according to claim 7, wherein said polyhedron comprises four planar surfaces.

9. The apparatus according to claim 1 further comprising:

a receiving area treated with an adhesive material,

said adhesive material allowing the attachment and removal of said chamber from said microscope slide.

10. The apparatus according to claim 9, wherein adhesive comprises a biocompatible acrylic material.

11. The apparatus according to claim 9, wherein said receiving area is treated with a hydrophobic backing to promote adhesive function.

12. The apparatus according to claim 9, wherein said receiving area is treated with a hydrophobic backing to form a reagent dam.

13. The apparatus according to claim 1 further comprising:

a liner configured to fit the inside walls of said chamber and having a neck portion that removably attaches to said opening of said chamber, as well as a flange portion that removably attaches to an edge of said bottom portion of said chamber; and

a shell that conforms to and fits over the outside walls of said chamber.

14. The apparatus according to claim 13, wherein said liner comprises a disposable polyethylene.

15. The apparatus according to claim 13, wherein said shell comprises polyethylene.

16. The apparatus according to claim 13, further comprising a means for securing said chamber onto said receiving area on said microscope slide by pressing on said shell to seal said flange against said receiving area.

17. An apparatus in accordance with claim 16, wherein the means for securing said chamber comprises a handle which engages a compression plate positioned over said chamber.

18. A method for depositing biological material in a cell deposition chamber comprising the steps of:

providing a cell deposition chamber having a footprint at a bottom portion of said chamber and defining an opening at a top portion of said chamber;

providing a microscope slide having a receiving area for a biological sample, said receiving area adapted to match the footprint of said cell deposition chamber;

treating said microscope slide with a chemical backing;

fitting said chamber with a liner, said liner having a neck that fits over said opening of said chamber, and a flange that fits over an edge of said bottom portion of said chamber;

placing said footprint of said chamber over said receiving area and securing said chamber on said microscope slide;

introducing biological material onto said microscope slide via said opening of said deposition chamber,

closing and sealing said opening.

19. A method according to claim 18, wherein said biological sample comprises cells in a suspension liquid.

20. A method according to claim 18, wherein said biological sample comprises organelles in a suspension liquid.

21. A method according to claim 18 further comprising the steps of:

performing an in vitro diagnostic testing of said biological sample;

opening said port; and

aspirating supernatant liquid of said biological sample through said port.

22. A method according to claim 21 further comprising the steps of:

closing and sealing said port at top of said chamber after aspirating said supernatant liquid;

placing a compression plate over said chamber;

actuating a handle to press said compression plate to seal said chamber onto said receiving area on said microscope slide.

23. The method according to claim 22, wherein said handle seals said chamber onto said slide in a cytocentrifugation bucket.