Novel fungal proteins and nucleic acids encoding same
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Abstract
Disclosed herein are fungal nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The novel leucine aminopeptidase (LAP) and other amino- and carboxypeptidases polypeptides, referred to herein as EXOX nucleic acids and proteins of the invention are useful in a variety of medical, research, and commercial applications.
83 Citations
66 Claims
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1. (canceled)
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2. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2, 3, 6, 21, 24, 27, 30, and 35.
- View Dependent Claims (15)
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3-14. -14. (canceled)
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16-41. -41. (canceled)
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42. An enzyme cocktail comprising
a) a dipeptidyl peptidase IV (DPP IV) comprising SEQ ID NO: - 35, a biologically active fragment thereof, a naturally occurring allelic variant thereof, or a sequence having at least 90% of identity, and
b) an aminopeptidase originating from a dermatophyte comprising the mature form of an amino acid sequence comprising either i) a leucine aminopeptidase (LAP) comprising SEQ ID NO;
3, a biologically active fragment thereof, a naturally occurring allelic variant thereof, and/or a sequence having at least 90% of identity;
orii) a leucine aminopeptidase (LAP) comprising SEQ ID NO;
6, a biologically active fragment thereof, a naturally occurring allelic variant thereof, and/or a sequence having at least 90% of identity. - View Dependent Claims (43, 44, 45, 46, 47, 48, 49, 50, 51, 56, 57, 58, 59, 60, 61)
- 35, a biologically active fragment thereof, a naturally occurring allelic variant thereof, or a sequence having at least 90% of identity, and
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52. A method of treating a pathological state in a mammal, comprising administering to the mammal an enzyme cocktail in an amount that is sufficient to alleviate the pathological state, wherein the enzyme cocktail comprises
a) a dipeptidyl peptidase IV (DPP IV) comprising SEQ ID NO: - 35, a biologically active fragment thereof, a naturally occurring allelic variant thereof, or a sequence having at least 90% of identity, and
b) an aminopeptidase originating from a dermatophyte comprising the mature form of an amino acid sequence comprising either i) a leucine aminopeptidase (LAP) comprising SEQ ID NO;
3, a biologically active fragment thereof, a naturally occurring allelic variant thereof, and/or a sequence having at least 90% of identity;
orii) a leucine aminopeptidase (LAP) comprising SEQ ID NO;
6, a biologically active fragment thereof, a naturally occurring allelic variant thereof, and/or a sequence having at least 90% of identity. - View Dependent Claims (53, 54, 55)
- 35, a biologically active fragment thereof, a naturally occurring allelic variant thereof, or a sequence having at least 90% of identity, and
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62. A composition for removing amino acids from the amino terminus of a protein comprising a dipeptidyl peptidase IV (DPPIV) of SEQ ID NO:
- 35, a biologically active fragment thereof, a naturally occurring allelic variants thereof, or a sequence having at least 90% of identity.
- View Dependent Claims (63, 64, 65, 66)
Specification