Systems and Methods for Multiple Analyte Detection
First Claim
1. A system for distribution of a biological sample, the system comprising:
- a substrate, wherein the substrate comprises a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber;
a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate; and
a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber, wherein the substrate is constructed of detection-compatible and assay-compatible materials.
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Abstract
Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
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Citations
65 Claims
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1. A system for distribution of a biological sample, the system comprising:
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a substrate, wherein the substrate comprises a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber;
a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate; and
a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber, wherein the substrate is constructed of detection-compatible and assay-compatible materials. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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50. A method for distribution of a biological sample, the method comprising:
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providing a base, wherein the base comprises a plurality of sample cavities containing a preloaded reagent therein, a sample introduction trench for each sample cavity, and a venting trench for each sample cavity, and wherein the base is constructed of detection-compatible and assay-compatible materials;
providing a film to adhere to the base to form a substrate, wherein the film and base form a plurality of sample chambers from each sample cavity, a sample introduction channel from each sample introduction trench, and a venting channel from each sample venting trench;
supplying the biological sample to the substrate;
filling the sample chambers with the biological sample via the sample introduction channels;
passing gas out of the substrate via at least one venting mechanism in the substrate; and
sealing the sample chambers to substantially prevent sample in the sample chambers from flowing out of the sample chambers. - View Dependent Claims (51, 52, 53, 54, 55, 56, 57)
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58. A system for distribution of a biological sample, the system comprising:
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means for distributing the sample to a plurality of sample chambers containing a preloaded reagent;
means for venting each of the sample chambers; and
means for sealing each of the sample chambers.
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59. A device for testing a biological sample, the device comprising:
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a substrate defining a plurality of distribution portions configured to distribute biological sample throughout the substrate, the plurality of distribution portions comprising a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber; and
a substance disposed in at least one of the distribution portions, the substance being configured to seal each sample chamber so as to substantially prevent sample disposed in each sample chamber from flowing out of each sample chamber during testing of the biological sample. - View Dependent Claims (60, 61, 62, 63, 64, 65)
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Specification