Correlation between the fucose content/galactose content ratio of anti-rhesus-d and anti-hla-dr antibodies and the adcc activity
First Claim
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1. A method for preparing a humanized or human chimeric monoclonal antibody, with high effector activity, comprising:
- a) producing and purifying monoclonal antibodies obtained from different sources selected from the group consisting of cells, plants and non-human animals, b) measuring the fucose content and the galactose content of the glycanic structures borne by the glycosylation site of the Fc region of said antibodies, and c) selecting antibodies for which the fucose content/galactose content ratio is less than or equal 0.6.
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Abstract
The present invention relates to monoclonal antibodies with high ADCC activity, characterised in having glycannic structures with a ratio (fucose content/galactose content) of 0.6 or less on the glycosylation site thereof in the Fc region. The invention also relates to pharmaceutical compositions, containing said monoclonal antibodies with high effector activity.
54 Citations
57 Claims
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1. A method for preparing a humanized or human chimeric monoclonal antibody, with high effector activity, comprising:
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a) producing and purifying monoclonal antibodies obtained from different sources selected from the group consisting of cells, plants and non-human animals, b) measuring the fucose content and the galactose content of the glycanic structures borne by the glycosylation site of the Fc region of said antibodies, and c) selecting antibodies for which the fucose content/galactose content ratio is less than or equal 0.6. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 28, 29, 31, 32, 33, 34, 35, 36, 37, 38)
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- 16. A method for increasing the effector activity of a composition of immunologically functional molecules, comprising the increase in galactose content and/or reduction in fucose content of the composition of molecules.
- 21. A cell derived from the YB2/0 cell line, in which at least one vector coding for an antibody molecule is introduced, said cell producing an antibody for which the fucose content/galactose content ratio of the oligosaccharides of the glycosylation site of the Fc region of the antibodies is less than or equal to 0.6.
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30. A pharmaceutical composition comprising at least 50% of a monoclonal antibody for which the glycanic structures borne by the glycosylation site of the Fc region have a fucose content/galactose content ratio less than 0.6.
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39. A method for preparing a human or humanized chimeric monoclonal antibody having low effector activity, comprising:
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a) producing and purifying monoclonal antibodies obtained from different sources selected from the group consisting of cells, plants, and non-human animals, b) measuring the fucose content and the galactose content of the glycanic structures borne by the glycosylation site of the Fc region of said antibodies, c) selecting antibodies for which the fucose content/galactose content ratio is larger than 0.6. - View Dependent Claims (40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 55, 56)
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- 51. A method for reducing the activity of a composition of immunologically functional molecules, comprising the increase in the fucose content and/or the reduction in the galactose content of said composition.
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57. A method for controlling the activity of a composition of immunologically functional molecules, comprising the regulation of the fucose content/galactose content ratio of the oligosaccharides of the glycosylation site of the Fc region of the antibodies.
Specification