DEVICES AND METHODS FOR PHARMACOKINETIC-BASED CELL CULTURE SYSTEM
First Claim
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1. A culture system of cells grown in a microscale culture device, comprising:
- a first chamber comprising a first cell type maintained under conditions that provide a value of at least one measurable parameter in vitro comparable to a value of at least one measurable parameter found in vivo;
a second chamber comprising a second cell type maintained under conditions that provide a value of at least one second measurable parameter comparable to a value of a second measurable parameter found in vivo;
at least one fluidic channel interconnecting the first and second chambers; and
an inlet and outlet for recirculation of culture medium.
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Abstract
Devices, in vitro cell cultures, systems, and methods are provided for microscale cell culture analogous (CCA) device.
65 Citations
56 Claims
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1. A culture system of cells grown in a microscale culture device, comprising:
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a first chamber comprising a first cell type maintained under conditions that provide a value of at least one measurable parameter in vitro comparable to a value of at least one measurable parameter found in vivo;
a second chamber comprising a second cell type maintained under conditions that provide a value of at least one second measurable parameter comparable to a value of a second measurable parameter found in vivo;
at least one fluidic channel interconnecting the first and second chambers; and
an inlet and outlet for recirculation of culture medium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A microscale culture device, comprising:
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a first microscale chamber having a geometry simulating a first in vivo interaction with culture medium, wherein the first chamber comprises a first inlet and a first outlet for flow of the culture medium;
a second microscale chamber having a geometry simulating a second in vivo interaction with the culture medium, wherein the second chamber comprises a second inlet and a second outlet for flow of the culture medium;
a microfluidic channel interconnecting the first and second chambers; and
at least one sensor for measuring at least one physiological event in the chambers. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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36. A microscale culture device comprising:
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a plurality of chambers connected by fluidic passages, each chamber having a geometry simulating parts of a living body; and
a pump, for circulating culture medium through the chambers to simulate the effects of compounds on the living body. - View Dependent Claims (37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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48. A microscale culture device, comprising:
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a lung simulating chamber;
a pump;
at least two chambers selected from the group consisting of a liver chamber that maintains cells under conditions that provide a value of at least one pharmacokinetic parameter in vitro comparable to a value of at least one pharmacokinetic parameter value found in vivo that is associated with a liver, a slowly perfused chamber, a rapidly perfused chamber and a fat chamber that maintains cells under conditions that provide a value of at least one pharmacokinetic parameter in vitro comparable to a value of at least one pharmacokinetic parameter value found in vivo that is associated with the function of fat; and
a plurality of microfluidic channels coupling the pump, and the at least two chambers serially or in parallel.
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49. A culture system of cells grown in a microscale culture device, comprising:
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a first chamber comprising a first cell type maintained under first selected conditions;
a second chamber comprising a second cell type maintained under second selected conditions, wherein the first and second chambers are interconnected by fluidic channels; and
an inlet and outlet for recirculation of culture medium. - View Dependent Claims (50, 51, 52, 53, 54, 55, 56)
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Specification