Treatment of skin, and wound repair, with thymosin beta 4

• US 20070015698A1
• Filed: 11/22/2005
• Published: 01/18/2007
• Est. Priority Date: 07/30/1998
• Status: Abandoned Application

• Current Assignee: RegeneRx Biopharmaceuticals, Inc., The United States of America, as represented by the Secretary, Department of Health and Human Services
• Original Assignee: RegeneRx Biopharmaceuticals, Inc., The United States of America, as represented by the Secretary, Department of Health and Human Services
• Inventors: Sosne, Gabriel, Goldstein, Allan, Kleinman, Hynda, Malinda, Katherine
• Application Number:
  US11/284,408
• Publication Number:
  US 20070015698A1
• Time in Patent Office: Days
• US Class Current: 514/8.100
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/35 having six-membered rings with one oxygen as the only ring hetero atom
• CPC Class Codes: A61K 31/366 having six-membered rings, e.g. delta-lactones
• CPC Class Codes: A61K 31/375 Ascorbic acid, i.e. vitamin C; Salts thereof
• CPC Class Codes: A61K 31/56 Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
• CPC Class Codes: A61K 31/57 substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone
• CPC Class Codes: A61K 38/1841 Transforming growth factor [TGF]
• CPC Class Codes: A61K 38/1866 Vascular endothelial growth factor [VEGF]
• CPC Class Codes: A61K 38/2292 Thymosin; Related peptides
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations

Abstract

Compositions and methods for treatment of skin utilizing thymosin β4.

Claims

1. A cosmetic composition for topical treatment of skin, comprising from 0. 1 ng/ml to 10 μ

g/ml of human thymosin β

4; and

a cosmetically acceptable vehicle.

2. The cosmetic composition according to claim 1, further comprising human transforming growth factor β

1 at a concentration from 5 pg to 1 ≢

g/ml.

3. The cosmetic composition according to claim 2, further comprising human vascular endothelial growth factor at a concentration from 1 pg/ml to 1 β

g/ml.

4. The composition according to claim 3, and further comprising one or more of estradiol;

progesterone;

pregnanalone;

coenzyme Q10;

methylsolanomethane (MSM);

copper peptide (copper extract);

plankton extract (phytosome);

tumor growth factor beta 1 (TGF-β

1);

glycolic acid;

kojic acid;

ascorbyl palmitate;

all trans retinol;

azaleic acid;

salicylic acid;

broparoestrol;

estrone;

adrostenedione; and

androstanediol.

5. The composition according to claim 3, wherein said composition further comprises a sunblock.

6. The composition according to claim 3, wherein said cosmetically acceptable vehicle is an oil in water, or water in oil emulsion.

7. A method for improving the appearance of the skin, the method comprising:

applying topically a cosmetic composition comprising from 0.1 ng/ml to 10 μ

g/ml of human thymosin β

4; and

a cosmetically acceptable vehicle.

8. The method according to claim 7, further comprising human transforming growth factor β

1 at a concentration from 5 pg to 1 μ

/ml.

9. The method according to claim 7, further comprising human vascular endothelial growth factor at a concentration from 1 pg/ml to 1 μ

/ml.

10. The method according to claim 9, wherein said skin is aged, photoaged, dry, lined or wrinkled skin.

11. The method according to claim 10, wherein said composition further comprises one or more of estradiol;

progesterone;

pregnanalone;

coenzyme Q10;

methylsolanomethane (MSM);

copper peptide (copper extract);

plankton extract (phytosome);

tumor growth factor beta 1 (TGF-β

1);

glycolic acid;

kojic acid;

ascorbyl palmitate;

all trans retinol;

azaleic acid;

salicylic acid;

broparoestrol;

estrone;

adrostenedione; and

androstanediols.

12. The method according to claim 10, wherein said composition further comprises a sunblock.

13. The method according to claim 10, wherein said cosmetically acceptable vehicle is an oil in water, or water in oil emulsion.

14. A cosmetic composition for topical treatment of skin, comprising from 0.1 ng/ml to 1 μ

/ml of human thymosin β

4; and

a cosmetically acceptable vehicle.

15. A cosmetic composition for topical treatment of skin, comprising from 0.1 ng/ml to 1 μ

g/ml of human thymosin β

4;

human transforming growth factor β

1 at a concentration from 5 pg to 1 μ

g/ml, and a cosmetically acceptable vehicle.

16. A cosmetic composition for topical treatment of skin, comprising from 0.1 ng/ml to 1 μ

g/ml of human thymosin β

4;

human vascular endothelial growth factor at a concentration from 1 pg/ml to 1 μ

g/ml and a cosmetically acceptable vehicle.

17. The composition according to claim 16, and further comprising one or more of estradiol, progesterone;

pregnanalone;

coenzyme Q10;

methylsolanomethane (MSM);

copper peptide (copper extract);

plankton extract (phytosome);

glycolic acid;

kojic acid;

ascorbyl palmitate;

all trans retinol;

azaleic acid;

salicylic acid;

broparoestrol;

estrone;

adrostenedione; and

androstanediol.

18. The composition according to claim 16, wherein said composition further comprises a sunblock.

19. The composition according to claim 16, wherein said cosmetically acceptable vehicle is an oil in water, or water in oil emulsion.

20. A method for improving the appearance of the skin, the method comprising:

applying topically a cosmetic composition comprising from 0.1 ng/ml to 1 μ

g/ml of human thymosin β

4; and

a cosmetically acceptable vehicle.

21. A method for improving the appearance of the skin, the method comprising:

applying topically a cosmetic composition comprising from 0.1 ng/ml to 1 μ

g/ml of human thymosin β

4, human transforming growth factor β

1 at a concentration from 5 pg to 1 μ

g/ml, and a cosmetically acceptable vehicle.

22. A method for improving the appearance of the skin, the method comprising:

applying topically a cosmetic composition comprising from 0.1 ng/ml to 1 μ

g/ml of human thymosin β

4, human vascular endothelial growth factor at a concentration from 1 pg/ml to 1 μ

g/ml, and a cosmetically acceptable vehicle.

23. The method according to claim 22, wherein said skin is aged, photoaged, dry, lined or wrinkled skin.

24. The method according to claim 23, wherein said composition further comprises one or more of estradiol;

progesterone;

pregnanalone;

coenzyme Q10;

methylsolanomethane (MSM);

copper peptide (copper extract);

plankton extract (phytbsome);

glycolic acid;

kojic acid;

ascorbyl palmitate;

all trans retinol;

azaleic acid;

salicylic acid;

broparoestrol;

estrone;

adrostenedione; and

androstanediols.

25. The method according to claim 23, wherein said composition further comprises a sunblock.

26. The method according to claim 23, wherein said cosmetically acceptable vehicle is an oil in water, or water in oil emulsion.

27. A method for preventing or treating damaged skin, the method comprising:

applying topically a composition comprising from 0.01 ng/ml to 1 μ

g/ml of human thymosin β

4; and

a vehicle.

28. The method according to claim 27, wherein the concentration of human thymosin β

4 is from at least about 0.1 ng/ml to 1 μ

g/ml.

29. A method for preventing or treating damaged skin, the method comprising:

applying topically a composition comprising from 0.01 ng/ml to 1 μ

g/ml of human thymosin β

4, human transforming growth factor β

1, and vehicle.

30. The method according to claim 29, wherein the concentration of human thymosin β

4 is from at least about 0.1 ng/ml to 1μ

g/ml.

31. A method for preventing or treating damaged skin, the method comprising:

applying topically a composition comprising from 0.01 ng/ml to 1 μ

g/ml of human thymosin β

4, human vascular endothelial growth factor, and a vehicle.

32. The method according to claim 31, wherein the concentration of human thymosin β

4 is from at least about 0.1 ng/ml to 1 μ

g/ml.

33. A composition, comprising from 0.01 ng/ml to 1 μ

g/ml of human thymosin β

4 and a vehicle.

34. The method according to claim 33, wherein the concentration of human thymosin β

4 is from at least about 0.1 ng/ml to 1 μ

g/ml.

35. The composition of claim 33, further comprising human transforming growth factor β

1.

36. The composition of claim 33, further comprising human vascular endothelial growth factor.

37. The composition according to claim 33, wherein the vehicle is an emulsion.

38. A method for treating epithelial tissue, the method comprising:

administering to a subject a composition comprising from 0.01 ng/ml to 1 μ

g/ml of human thymosin β

4 and a vehicle.

39. The method of according to claim 38, wherein the tissue is skin.

40. The method of claim 38, wherein said composition further comprises human transforming growth factor β

1.

41. The method of claim 38, wherein said composition further comprises human vascular endothelial growth factor.

42. The method according to claim 39, wherein the skin is aged, photoaged, dry, lined or wrinkled skin.

43. The method of claim 38, wherein the vehicle is an emulsion.

44. A method for improving appearance of skin, the method comprising:

administering to a subject a composition comprising from 0.01 ng/ml to 1 μ

g/ml of human thymosin β

4; and

a vehicle.

45. The method according to claim 44, wherein the concentration of human thymosin β

4 is from at least about 0.1 ng/ml to 1 μ

g/ml.

46. The method of claim 44, wherein said composition further comprises human transforming growth factor β

1.

47. The method of claim 44, wherein said composition further comprises human vascular endothelial growth factor.