Compounds for inhibiting diseases and preparing cells for transplantation

• US 20070015737A1
• Filed: 11/02/2005
• Published: 01/18/2007
• Est. Priority Date: 07/09/1999
• Status: Abandoned Application

• Current Assignee: Bellus Health (Interational) Limited
• Original Assignee: Isis Innovation Limited, Neurochem (International) Limited, Queen's University At Kingston, University of British Columbia, The Governing Council of the University of Toronto (University of Toronto)
• Inventors: Migneault, David, Szarek, Walter, Fraser, Paul, Clark, Anne, Verchere, Bruce, Gupta, Ajay
• Application Number:
  US11/265,537
• Publication Number:
  US 20070015737A1
• Time in Patent Office: Days
• US Class Current: 514/114
• CPC Class Codes: A61K 31/00 Medicinal preparations containing organic active ingredients
• CPC Class Codes: A61K 31/185 Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids hydroxamic acids A61K31/16; peroxy acids A61K31/327
• CPC Class Codes: A61K 31/194 having two or more carboxyl groups, e.g. succinic, maleic or phthalic acid
• CPC Class Codes: A61K 31/44 Non condensed pyridines; Hydrogenated derivatives thereof
• CPC Class Codes: A61K 31/47 Quinolines; Isoquinolines
• CPC Class Codes: A61K 31/472 Non-condensed isoquinolines, e.g. papaverine
• CPC Class Codes: A61K 31/662 Phosphorus acids or esters thereof having P—C bonds, e.g. foscarnet, trichlorfon
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 3/00 Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: C07K 14/4711 Alzheimer's disease; Amyloid plaque core protein

Abstract

Methods and compositions which are useful in the treatment of amyloidosis. In particular, methods and compositions are provided for inhibiting, preventing and treating amyloid depositions, e.g. in pancreatic islets, wherein the amyloidotic deposits are islet amyloid polypeptide (IAPP)-associated amyloid deposition or deposits. The methods of the invention involved administering to a subject a therapeutic compound which inhibits IAPP-associated amyloid deposits. Accordingly, the compositions and method of the invention are useful for inhibiting IAPP-associated amyloidosis in disorders in which such amyloid deposition occurs, such an diabetes. The invention also provides a process for the preparation of cells suitable for transplantation into a mammal, which cells are capable of forming fibrils, said process comprising contacting the cells with an inhibitor of fibril formation. In particular the process prepares cells for use in a method of treating diabetes. Also provided are a culture medium comprising the inhibitor and cells for transplantation.

Claims

1-21. -21. (canceled)

22. A method for the preparation of cells suitable for treating a subject having amyloid deposits, said method comprising contacting the cells in vitro with an inhibitor of amyloid deposit formation, such that amyloid deposit formation is inhibited therein.

23. The method according to claim 22, wherein said inhibitor causes breakdown of amyloid deposits, the deposits having been formed by said cells prior to said contacting.

24. The method according to claim 22, wherein said cells are cultured in the presence of the inhibitor.

27. The method according to claim 22, wherein said inhibitor comprises a compound selected from the group consisting of:

(i) 3-(3-hydroxy-1-propyl)amino-1-propanesulfonic acid

HOCH₂CH₂CH₂NHCH₂CH₂CH₂SO₃H;

(ii) DL-2-amino-5-phosphovaleric acid

(iii) 1,2,3,4-tetrahydroisoquinoline

(iv) cyclohexylsulfamic acid

(v) O-phospho-L-serine

(vi) hexafluoroglutaric acid

(vii) 8-methoxyquinoline-5-sulfonic acid

(viii) 4-phenyl-1-(3′

-sulfopropyl)-1,2,3,6-tetrahydropyridine

(ix) 3-amino-2-hydroxy-1-propanesulfonic acid

(x) 3-dimethylamino-1-propanesulfonic acid

Me₂NCH₂CH₂CH₂SO₃H;

and pharmaceutically acceptable esters, acids, and salts thereof.

32. A culture medium or a culture medium pre-mix comprising a compound as defined in claim 53.

33. A culture of cells in which the culture medium is as defined in claim 32.

34. A culture according to claim 33 in which the cells are islet cells.

35. Ex vivo cells prepared by the method according to claim 22.

36. Ex vivo cells according to claim 35, wherein said cells are in a preparation comprising an inhibitor, wherein said inhibitor comprises a compound selected from the group consisting of:

(i) 3-(3-hydroxy-1-propyl)amino-1-propanesulfonic acid;

(ii) DL-2-amino-5-phosphovaleric acid;

(iii) 1,2,3,4-tetrahydroisoguinoline;

(iv) cyclohexylsulfamic acid;

(v) O-phospho-L-serine;

(vi) hexafluoroglutaric acid;

(vii) 8-methoxyquinoline-5-sulfonic acid;

(viii) 4-phenyl-1-(3′

-sulfopropyl)-1,2,3,6-tetrahydropyridine;

(ix) 3-amino-2-hydroxy-1-propanesulfonic acid;

(x) 3-dimethylamino-1-propanesulfonic acid;

and pharmaceutically acceptable esters, acids, and salts thereof.

39. Ex vivo cells according to claim 35 which are islet cells for use in a method of treating diabetes.

41. A pharmaceutical composition comprising a cell according to claim 35, and a pharmaceutically acceptable carrier or diluent.

42. Use of an inhibitor as defined in claim 53 in the manufacture of a medicament for inhibiting amyloid deposit formation by, or breaking amyloid deposits down in, a transplanted cell preparation.

43. A vessel for containing a culture of cells, wherein said vessel is coated with a compound as defined in claim 53.

44. A kit for culturing cells comprising a culture medium or culture medium pre-mix as defined in claim 32.

46. A method of identifying an inhibitor that can be used to prepare cells for transplantation in the method according to claim 22, comprising contacting a candidate substance with a mammalian cell and determining whether the candidate substance inhibits the formation of fibrils or causes the breakdown of fibrils, indicating that the substance is an inhibitor that can be used in said method.

47. A method of identifying an inhibitor that can be used to prepare cells for transplantation in the method according to claim 22, comprising contacting a candidate substance with a protein capable of forming fibrils, or with a fibril, and determining whether the substance inhibits the formation of the protein into a fibril, or whether the substance causes the breakdown of fibrils, indicating that the substance can be used in said method.

51. A method of inhibiting fibril formation by, or breaking fibrils down in, a transplanted cell preparation comprising administering an inhibitor as defined in claim 53 to a patient who has received a transplant of cells as defined in claim 35.

53. The method of claim 22 wherein said inhibitor of amyloid deposit formation is a compound of the formula

wherein k, m, t, p and q are independently 0 or 1;

n is an integer from 0 to 3;

C is a carbon;

N is a nitrogen;

W is hydrogen or an anionic group at physiological pH;

Y is an anionic group at physiological pH;

R¹ and R² are independently hydrogen, alkyl, an anionic group at physiological pH, or R¹ and R², taken together with the nitrogen to which they are attached, may form an unsubstituted or substituted heterocycl having from 3 to 7 atoms in the heterocyclic ring;

R³ is hydrogen, halogen, thiol or hydroxyl;

R⁴, R⁵, and R⁶ are independently hydrogen or halogen; and

A is hydrogen or C₁ to C₆ alkyl;

or a pharmaceutically acceptable ester, acid or salt thereof.

54. The method of claim 22 wherein said inhibitor of amyloid deposit formation is a compound of the formula

wherein C is a carbon;

N is a nitrogen;

H is a hydrogen;

A¹, A², A³, A⁴, A⁵ and A⁶ are independently alkyl, O, S, or —

NH;

m and n (for each individual A group) are independently 0 or 1;

p, q and 1 are independently 0, 1, or 2;

R⁷, R⁸, R⁹, R¹, R¹¹, R¹², and each R¹⁴ are independently hydrogen, alkyl, alicyclyl, heterocycyl or aryl, each R¹³ is independently hydrogen, alkyl, alicyclyl, heterocycyl, aryl or an anionic group, and adjacent R groups (e.g., R⁷ and R⁸) may form an unsubstituted or substituted '"'"'0 cyclic or heterocyclic ring.

25-26. -26. (canceled)

28-31. -31. (canceled)

37-38. -38. (canceled)

40. (canceled)

45. (canceled)

48-50. -50. (canceled)

52. (canceled)