Miniaturized fluid delivery and analysis system
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Abstract
The present invention provides a method for combining a fluid delivery system with an analysis system for performing immunological or other chemical of biological assays. The method comprises a miniature plastic fluidic cartridge containing a reaction chamber with a plurality of immobilized species, a capillary channel, and a pump structure along with an external linear actuator corresponding to the pump structure to provide force for the fluid delivery. The plastic fluidic cartridge can be configured in a variety of ways to affect the performance and complexity of the assay performed.
89 Citations
17 Claims
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1-11. -11. (canceled)
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12. A method of performing immunological assays of a fluid sample comprising a plurality of bio-molecules at unknown concentrations, comprising the steps of:
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(a) placing said fluid sample into a sample reservoir defined in a fluidic cartridge;
(b) placing a wash buffer in a wash buffer reservoir defined in said fluidic cartridge;
(c) providing an air purge reservoir in said fluidic cartridge that remains empty to provide air purging;
(d) placing a secondary antibody conjugate in an antibody reservoir defined in said fluidic cartridge;
(e) placing a substrate solution specific to a secondary antibody conjugate in a substrate reservoir defined in said fluidic cartridge;
(f) pumping, by a pump structure, each of said fluid sample, said wash buffer, said substrate solution, and said secondary antibody conjugate to a waste reservoir through a first reaction chamber;
(g) providing a second reaction chamber for negative control and isolating said second reaction chamber from said fluid sample of said first reservoir by a check valve; and
(h) confirming results of immunoassay by optical measurements. - View Dependent Claims (13, 14, 15, 16, 17)
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Specification