Miniaturized fluid delivery and analysis system
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Abstract
The present invention provides a method for combining a fluid delivery system with an analysis system for performing immunological or other chemical of biological assays. The method comprises a miniature plastic fluidic cartridge containing a reaction chamber with a plurality of immobilized species, a capillary channel, and a pump structure along with an external linear actuator corresponding to the pump structure to provide force for the fluid delivery. The plastic fluidic cartridge can be configured in a variety of ways to affect the performance and complexity of the assay performed.
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Citations
19 Claims
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1-11. -11. (canceled)
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12. A method of performing DNA hybridization analysis, comprising the steps of:
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(a) immobilizing a plurality of DNA probes in a reaction chamber defined in a fluidic cartridge;
(b) placing a fluid sample containing one or more populations of fluorescently tagged, amplified DNA of unknown sequence in a sample reservoir defined in said fluidic cartridge;
(c) placing a first stringency wash buffer in a first wash buffer reservoir defined in said fluidic cartridge;
(d) placing a second stringency wash buffer in a second wash buffer reservoir defined in said fluidic cartridge;
(e) maintaining a reaction chamber in a constant temperature;
(f) pumping said fluid sample from said sample reservoir to a circulation reservoir defined in said fluidic cartridge and circulating said fluid sample through said reaction chamber for a predetermined hybridization time;
(g) pumping out said fluid sample from said circulation reservoir and said reaction chamber;
(h) pumping said first stringency wash buffer from said first wash buffer reservoir to said circulation reservoir and circulating said first stringency wash buffer through said reaction chamber for a first predetermined wash time;
(i) pumping out said first stringency wash buffer from said circulation reservoir and said reaction chamber;
(j) pumping said second stringency wash buffer from said second wash buffer reservoir to said circulation reservoir and circulating said second stringency wash buffer through said reaction chamber for a second predetermined wash time;
(k) pumping out said second stringency wash buffer from said circulation reservoir and said reaction chamber; and
(i) achieving a DNA hybridization. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
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Specification