DEVICES AND METHODS FOR PHARMACOKINETIC-BASED CELL CULTURE SYSTEM
First Claim
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1. A pharmacokinetic-based microscale culture device, comprising:
- a first microscale chamber dimensioned to maintain biological material under conditions that provide a value of at least one pharmacokinetic parameter in vitro that is comparable to a value of at least one pharmacokinetic parameter obtained in vivo, wherein the first microscale chamber comprises a first inlet and a first outlet for flow of fluid;
a second microscale chamber dimensioned to maintain biological material under conditions that provide a value of at least one pharmacokinetic parameter in vitro that is comparable to a value of at least one pharmacokinetic parameter obtained in vivo, wherein the second microscale chamber comprises a second inlet and a second outlet for flow of fluid;
a microfluidic channel interconnecting the first and second microscale chambers wherein the microfluidic channel is dimensioned to transport the fluid; and
a debubbler that is configured to remove bubbles from the fluid.
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Abstract
Devices, in vitro cell cultures, systems, and methods are provided for microscale cell culture analogous (CCA) device.
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Citations
43 Claims
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1. A pharmacokinetic-based microscale culture device, comprising:
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a first microscale chamber dimensioned to maintain biological material under conditions that provide a value of at least one pharmacokinetic parameter in vitro that is comparable to a value of at least one pharmacokinetic parameter obtained in vivo, wherein the first microscale chamber comprises a first inlet and a first outlet for flow of fluid;
a second microscale chamber dimensioned to maintain biological material under conditions that provide a value of at least one pharmacokinetic parameter in vitro that is comparable to a value of at least one pharmacokinetic parameter obtained in vivo, wherein the second microscale chamber comprises a second inlet and a second outlet for flow of fluid;
a microfluidic channel interconnecting the first and second microscale chambers wherein the microfluidic channel is dimensioned to transport the fluid; and
a debubbler that is configured to remove bubbles from the fluid.
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2. A device comprising:
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at least one microscale feature dimensioned to maintain biological material under conditions that provide a value of at least one pharmacokinetic parameter in vitro that is comparable to a value of at least one pharmacokinetic parameter found in vivo; and
a debubbler that is configured to remove bubbles from fluid that flows in or through the device. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A method of culturing biological material comprising:
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maintaining biological material with a first microscale chamber under conditions that provide a value of at least one pharmacokinetic parameter in vitro that is comparable to a value of at least one pharmacokinetic parameter obtained in vivo, wherein the first microscale chamber comprises a first inlet and a first outlet for flow of fluid;
maintaining biological material with a second microscale chamber under conditions that provide a value of at least one pharmacokinetic parameter in vitro that is comparable to a value of at least one pharmacokinetic parameter obtained in vivo, wherein the second microscale chamber comprises a second inlet and a second outlet for flow of fluid;
interconnecting the first and second microscale chambers with a microfluidic channel wherein the microfluidic channel is dimensioned to transport the fluid; and
removing bubbles from the fluid.
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18. A method comprising:
maintaining biological material with at least one microscale feature under conditions that provide a value of at least one pharmacokinetic parameter in vitro that is comparable to a value of at least one pharmacokinetic parameter found in vivo; and
removing bubbles from fluid that flows in or through the device.- View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A method comprising:
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forming a microscale feature that is dimensioned to maintain biological material under conditions that provide a value of at least one pharmacokinetic parameter in vitro that is comparable to a value of at least one pharmacokinetic parameter found in vivo; and
adding, forming, or providing for a debubbler that is configured to remove bubbles from fluid that flows in or through the device. - View Dependent Claims (32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42)
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43. A device comprising:
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a microscale chamber comprising a biological material and a geometry simulating parts of a living body, wherein the microscale chamber is configured to substantially mimic at least one measurable in vivo condition in vivo; and
a debubbler that is configured to remove bubbles from fluid that flows in or through the device.
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Specification