METHOD FOR OBTAINING PURIFIED RNA FROM VIABLE OOCYSTS

• US 20070020661A1
• Filed: 07/25/2006
• Published: 01/25/2007
• Est. Priority Date: 09/12/2000
• Status: Active Grant

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Inventors: STULL, Paul, WEISBURG, William, CUNNINGHAM, Melissa
• Granted Patent: US 7,585,631 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6893 for protozoa
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention describes novel oligonucleotides targeted to nucleic acid sequences derived from Crytosporidium organisms, and Crytosporidium parvum organisms in particular, which are useful for determining the presence of Cryptosporidium organisms in a test sample. The oligonucleotides of the present invention include hybridization assay probes, helper probes and amplification primers. The present invention further describes a novel method for obtaining purified ribonucleic acid from viable oocysts.

Claims

3-1. The method of claim 1, wherein said centrifuging step is performed at about 7000 revolutions per minute for approximately 5 minutes.