Methods and compositions for amplifying nucleic acids
First Claim
Patent Images
1. A method of amplifying a plurality of target nucleic acid sequences comprising:
- forming a reaction composition comprising (a) a plurality of target nucleic acid sequences, (b) at least one set of primers, and (c) at least one polymerase;
incubating the reaction composition under conditions wherein one or more of the plurality of target nucleic acid sequences are amplified;
wherein at least one of the at least one set of primers comprises a plurality of primers, wherein each primer of the plurality of primers comprises at least one designed portion and at least one random portion;
wherein one of the at least one designed portions consists of two nucleotides at the 5′
portion of the primer.
6 Assignments
0 Petitions
Accused Products
Abstract
Methods and compositions for amplifying nucleic acids are provided.
15 Citations
42 Claims
-
1. A method of amplifying a plurality of target nucleic acid sequences comprising:
-
forming a reaction composition comprising (a) a plurality of target nucleic acid sequences, (b) at least one set of primers, and (c) at least one polymerase;
incubating the reaction composition under conditions wherein one or more of the plurality of target nucleic acid sequences are amplified;
wherein at least one of the at least one set of primers comprises a plurality of primers, wherein each primer of the plurality of primers comprises at least one designed portion and at least one random portion;
wherein one of the at least one designed portions consists of two nucleotides at the 5′
portion of the primer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
-
-
19. A composition for amplifying a plurality of target nucleic acid sequences comprising:
-
(a) a plurality of target nucleic acid sequences;
(b) at least one set of primers; and
(c) at least one DNA polymerase;
wherein at least one of the at least one set of primers comprises a plurality of primers, wherein each primer of the plurality of primers comprises at least one designed portion and at least one random portion;
wherein one of the at least one designed portions consists of two nucleotides at the 5′
portion of the primer. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
-
-
32. A method of amplifying a plurality of target nucleic acid sequences comprising:
-
forming a reaction composition comprising (a) a plurality of target nucleic acid sequences comprising a first position and a second position, (b) at least one set of primers, and (c) at least one DNA polymerase;
incubating the reaction composition under amplification conditions wherein one or more of the plurality of target nucleic acid sequences are amplified to form amplification products;
wherein at least one of the at least one set of primers comprises a plurality of primers, wherein each primer of the plurality of primers comprises at least one designed portion and at least one random portion;
wherein the at least one set of primers produces a first amplification product comprising the first position and a second amplification product comprising the second position;
wherein the amount of first amplification product produced with the at least one set of primers is greater than the amount of first amplification product produced with random primers incubated under the amplification conditions with the plurality of target nucleic acid sequences and the at least one polymerase; and
wherein the amount of second amplification product produced with the at least one set of primers is greater than the amount of second amplification product produced with random primers incubated under the amplification conditions with the plurality of target nucleic acid sequences and the at least one polymerase. - View Dependent Claims (33, 34, 35)
-
-
36. A method of determining similarity between a plurality of target nucleic acid sequences from one or more sources and one or more reference sequences comprising:
-
forming a reaction composition comprising (a) a plurality of target nucleic acid sequences from one or more sources, (b) at least one set of primers, and (c) at least one DNA polymerase;
incubating the reaction composition under conditions wherein one or more of the plurality of target nucleic acid sequences are amplified to form amplification products;
wherein at least one of the at least one set of primers comprises a plurality of primers, wherein each primer of the plurality of primers comprises at least one designed portion and at least one random portion;
wherein one of the at least one designed portions consists of two nucleotides at the 5′
portion of the primer;
comparing sequence information of the amplification products with sequence information of the one or more reference sequences to identify similarities and differences between the sequence information of the amplification products and the sequence information of the one or more reference sequences;
wherein each of the one or more reference sequences comprises a plurality of nucleic acid sequences;
determining the similarity between a plurality of target nucleic acid sequences from one or more sources and the one or more reference sequences based on the identified similarities and differences. - View Dependent Claims (37, 38, 39)
-
-
40. A method of amplifying a plurality of target nucleic acid sequences comprising:
-
forming a reaction composition comprising (a) a plurality of target nucleic acid sequences, (b) at least one set of primers, and (c) at least one DNA polymerase;
incubating the reaction composition under conditions wherein one or more of the plurality of target nucleic acid sequences are amplified;
wherein at least one of the at least one set of primers comprises a plurality of primers, wherein each primer of the plurality of primers comprises at least one designed portion and at least one random portion;
wherein one of the at least one designed portions comprises the 5′
portion of the primer.
-
-
41. A method of amplifying a plurality of target nucleic acid sequences comprising:
-
forming a reaction composition comprising (a) a plurality of target nucleic acid sequences, (b) at least one set of primers, and (c) at least one DNA polymerase;
incubating the reaction composition under conditions wherein one or more of the plurality of target nucleic acid sequences are amplified;
wherein at least one of the at least one set of primers comprises a plurality of primers, wherein each primer of the plurality of primers comprises at least one designed portion and at least one random portion;
wherein one of the at least one designed portions comprises the 3′
portion of the primer.
-
-
42. A method of amplifying a plurality of target nucleic acid sequences comprising:
-
forming a reaction composition comprising (a) a plurality of target nucleic acid sequences, (b) at least one set of primers, and (c) at least one DNA polymerase;
incubating the reaction composition under conditions wherein one or more of the plurality of target nucleic acid sequences are amplified;
wherein at least one of the at least one set of primers comprises a plurality of primers, wherein each primer of the plurality of primers comprises at least one designed portion and at least one random portion;
wherein at least one of the at least one designed portions is not a constant portion.
-
Specification