Hyaluronan synthases and methods of making and using same

• US 20070020737A1
• Filed: 07/28/2006
• Published: 01/25/2007
• Est. Priority Date: 12/03/2001
• Status: Abandoned Application

• Current Assignee: Paul Deangelis, Paul Weigel, Philip Pummill
• Original Assignee: Paul Deangelis, Paul Weigel, Philip Pummill
• Inventors: DeAngelis, Paul, Pummill, Philip, Weigel, Paul
• Application Number:
  US11/495,989
• Publication Number:
  US 20070020737A1
• Time in Patent Office: Days
• US Class Current: 435/85
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)
• CPC Class Codes: C12P 19/26 Preparation of nitrogen-containing carbohydrates

Abstract

A functionally active hyaluronan synthase having at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase such that the functionally active hyaluronan synthase has an altered enzymatic activity as compared to the corresponding functionally active native hyaluronan synthase is disclosed. The altered enzymatic activity may result in increased or decreased activity when compared to the corresponding native hyaluronan synthase, or the altered enzymatic activity may result in production of hyaluronan having an average molecular mass that is greater than or less than an average molecular mass of hyaluronan produced by the corresponding native hyaluronan synthase. Methods of producing hyaluronic acid utilizing a recombinant host cell having an expression construct encoding the functionally active hyaluronan synthase with altered enzymatic activity are also disclosed.

Claims

1. A functionally active hyaluronan synthase having an altered enzymatic activity as compared to a corresponding functionally active native hyaluronan synthase, wherein the functionally active hyaluronan synthase has at least one modified amino acid residue therein as compared to the corresponding functionally active native hyaluronan synthase.

2. The functionally active hyaluronan synthase of claim 1, wherein the corresponding functionally active native hyaluronan synthase is selected from the group consisting of spHAS, seHAS, suHAS and pmHAS.

3. The functionally active hyaluronan synthase of claim 2, wherein the corresponding functionally active native hyaluronan synthase has an amino acid sequence essentially as set forth in at least one of SEQ ID NOS:

2, 4, 6, 8, 10, 12 and 14.

4. The functionally active hyaluronan synthase of claim 1, wherein the at least one modified amino acid residue is selected from the group consisting of an asparagine, a cysteine, a glycine, a lysine, a phenylalanine, a threonine, and combinations thereof.

5. The functionally active hyaluronan synthase of claim 1, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has an amino acid sequence essentially as set forth in at least one of SEQ ID NOS:

93-160.

6. The functionally active hyaluronan synthase of claim 1, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has an increased enzymatic activity when compared to the corresponding functionally active native hyaluronan synthase.

7. The functionally active hyaluronan synthase of claim 1, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has a decreased enzymatic activity when compared to the corresponding functionally active native hyaluronan synthase.

8. The functionally active hyaluronan synthase of claim 1, wherein the functionally active hyaluronan synthase having an altered enzymatic activity produces hyaluronic acid having an average molecular mass that is less than an average molecular mass of hyaluronic acid produced by the corresponding functionally active native hyaluronan synthase.

9. The functionally active hyaluronan synthase of claim 1, wherein the functionally active hyaluronan synthase having an altered enzymatic activity produces hyaluronic acid having an average molecular mass that is greater than an average molecular mass of hyaluronic acid produced by the corresponding functionally active native hyaluronan synthase.

10. A recombinant host cell having a functionally active hyaluronan synthase having an altered enzymatic activity as compared to a corresponding functionally active native hyaluronan synthase incorporated therein such that the host cell is capable of producing hyaluronan, wherein the functionally active hyaluronan synthase has at least one modified amino acid residue therein as compared to the corresponding functionally active native hyaluronan synthase.

11. The recombinant host cell of claim 10, wherein the corresponding functionally active native hyaluronan synthase is selected from the group consisting of spHAS, seHAS, suHAS and pmHAS.

12. The recombinant host cell of claim 11, wherein the corresponding functionally active native hyaluronan synthase has an amino acid sequence essentially as set forth in at least one of SEQ ID NOS:

2, 4, 6, 8, 10, 12 and 14.

13. The recombinant host cell of claim 10, wherein the at least one modified amino acid residue is selected from the group consisting of an asparagine, a cysteine, a glycine, a lysine, a phenylalanine, a threonine, and combinations thereof.

14. The recombinant host cell of claim 10, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has an amino acid sequence essentially as set forth in at least one of SEQ ID NOS:

93-160.

15. The recombinant host cell of claim 10, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has an increased enzymatic activity when compared to the corresponding functionally active native hyaluronan synthase.

16. The recombinant host cell of claim 10, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has a decreased enzymatic activity when compared to the corresponding functionally active native hyaluronan synthase.

17. The recombinant host cell of claim 10, wherein the functionally active hyaluronan synthase having an altered enzymatic activity produces hyaluronic acid having an average molecular mass that is less than an average molecular mass of hyaluronic acid produced by the corresponding functionally active native hyaluronan synthase.

18. The recombinant host cell of claim 10, wherein the functionally active hyaluronan synthase having an altered enzymatic activity produces hyaluronic acid having an average molecular mass that is greater than an average molecular mass of hyaluronic acid produced by the corresponding functionally active native hyaluronan synthase.

19. The recombinant host cell of claim 10, wherein the recombinant host cell is a Bacillus cell.

20. The recombinant host cell of claim 19, wherein the recombinant host cell is selected from the group consisting of Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus metaterium, Bacillus pumilus, Bacillus stearothermophilus, Bacillus subtilis and Bacillus thuringienisis.

21. A method of providing a functionally active hyaluronan synthase having an altered enzymatic activity as compared to a corresponding functionally active native hyaluronan synthase, comprising the steps of:

providing a hyaluronan synthase; and

modifying at least one amino acid residue of the hyaluronan synthase to provide a functionally active hyaluronan synthase having an altered enzymatic activity.

22. The method of claim 21, wherein the corresponding functionally active native hyaluronan synthase is selected from the group consisting of spHAS, seHAS, suHAS and pmHAS.

23. The method of claim 22, wherein the corresponding functionally active native hyaluronan synthase has an amino acid sequence essentially as set forth in at least one of SEQ ID NOS:

2, 4, 6, 8, 10, 12 and 14.

24. The method of claim 21 wherein, in the step of modifying at least one amino acid residue, the at least one modified amino acid residue is selected from the group consisting of an asparagine, a cysteine, a glycine, a lysine, a phenylalanine, a threonine, and combinations thereof.

25. The method of claim 21, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has an amino acid sequence essentially as set forth in at least one of SEQ ID NOS:

93-160.

26. The method of claim 21, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has an increased enzymatic activity when compared to the corresponding functionally active native hyaluronan synthase.

27. The method of claim 21, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has a decreased enzymatic activity when compared to the corresponding functionally active native hyaluronan synthase.

28. The method of claim 21, wherein the functionally active hyaluronan synthase having an altered enzymatic activity produces hyaluronic acid having an average molecular mass that is less than an average molecular mass of hyaluronic acid produced by the corresponding functionally active native hyaluronan synthase.

29. The method of claim 21, wherein the functionally active hyaluronan synthase having an altered enzymatic activity produces hyaluronic acid having an average molecular mass that is greater than an average molecular mass of hyaluronic acid produced by the corresponding functionally active native hyaluronan synthase.

30. A method for producing hyaluronic acid, comprising the steps of:

providing a recombinant host cell having at least one expression construct comprising a hyaluronan synthase gene encoding a functionally active hyaluronan synthase incorporated therein such that the host cell is capable of producing hyaluronan, wherein the functionally active hyaluronan synthase has at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase such that the functionally active hyaluronan synthase has an altered enzymatic activity as compared to the corresponding functionally active native hyaluronan synthase; and

culturing the host cell under conditions appropriate for the production of hyaluronic acid.

31. The method of claim 30, wherein the corresponding functionally active native hyaluronan synthase is selected from the group consisting of spHAS, seHAS, suHAS and pmHAS.

32. The method of claim 31, wherein the corresponding functionally active native hyaluronan synthase has an amino acid sequence essentially as set forth in at least one of SEQ ID NOS:

2, 4, 6, 8, 10, 12 and 14.

33. The method of claim 30, wherein the at least one modified amino acid residue is selected from the group consisting of an asparagine, a cysteine, a glycine, a lysine, a phenylalanine, a threonine, and combinations thereof.

34. The method of claim 30, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has an amino acid sequence essentially as set forth in at least one of SEQ ID NOS:

93-160.

35. The method of claim 30, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has an increased enzymatic activity when compared to the corresponding functionally active native hyaluronan synthase.

36. The method of claim 30, wherein the functionally active hyaluronan synthase having an altered enzymatic activity has a decreased enzymatic activity when compared to the corresponding functionally active native hyaluronan synthase.

37. The method of claim 30, wherein the functionally active hyaluronan synthase having an altered enzymatic activity produces hyaluronic acid having an average molecular mass that is less than an average molecular mass of hyaluronic acid produced by the corresponding functionally active native hyaluronan synthase.

38. The method of claim 30, wherein the functionally active hyaluronan synthase having an altered enzymatic activity produces hyaluronic acid having an average molecular mass that is greater than an average molecular mass of hyaluronic acid produced by the corresponding functionally active native hyaluronan synthase.

39. The method of claim 30, further comprising the step of separating the hyaluronic acid from the host cell.

40. The method of claim 30, wherein the recombinant host cell further comprises at least one gene encoding an enzyme for synthesis of a hyaluronic acid sugar precursor.

41. The method of claim 30, wherein the at least one gene encoding an enzyme for synthesis of a hyaluronic acid sugar precursor is selected from the group consisting of a pyrophosphorylase, a transferase, a mutase, a dehydrogenase, an epimerase capable of increasing production of UDP-GlcNAc or UDP-GlcUA, and combinations thereof.

42. The method of claim 30, wherein nutrients utilized for a hyaluronic acid sugar precursor biosynthetic pathway are supplied to the host cell.

43. The method of claim 30, wherein the recombinant host cell is a Bacillus cell.

44. The method of claim 43, wherein the recombinant host cell is selected from the group consisting of Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillus clausii, Bacillus coagulans, Bacillus firmus, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus metaterium, Bacillus pumilus, Bacillus stearothermophilus, Bacillus subtilis and Bacillus thuringienisis.