Nucleic acid amino acid sequences relating to Streptococcus pneumoniae for diagnostics and therapeutics

• US 20070021601A1
• Filed: 09/20/2006
• Published: 01/25/2007
• Est. Priority Date: 07/02/1997
• Status: Active Grant

• Current Assignee: Chad Houseweart, David Bush, Lynn Doucette-Stamm, Qiandong Zeng, Timothy Opperman
• Original Assignee: Chad Houseweart, David Bush, Lynn Doucette-Stamm, Qiandong Zeng, Timothy Opperman
• Inventors: Doucette-Stamm, Lynn, Zeng, Qiandong, Houseweart, Chad, Bush, David, Opperman, Timothy
• Granted Patent: US 7,834,166 B2
• Time in Patent Office: Days
• US Class Current: 536/23.700
• CPC Class Codes: A61K 2039/53 DNA (RNA) vaccination
• CPC Class Codes: A61K 31/7052 having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/092 Streptococcus
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: C07H 21/02 with ribosyl as saccharide radical
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C07K 14/315 from Streptococcus (G), e.g. Enterococci
• CPC Class Codes: C07K 14/3156 from Streptococcus pneumoniae (Pneumococcus) Streptokinase C07K14/3153

Abstract

The invention provides isolated polypeptide and nucleic acid sequences derived from Streptococcus pneumoniae that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.

Claims

1. An isolated nucleic acid encoding a S. pneumoniae polypeptide having at least 98% identity to SEQ ID NO:

4360, wherein the isolated nucleic acid encodes a polypeptide that provides protective immunity against an infection by S. pneumoniae.

2. A recombinant expression vector comprising the nucleic acid of claim 1 operably linked to a transcription regulatory element.

3. A cell comprising the recombinant expression vector of claim 2.

4. An isolated nucleic acid having at least 99% identity to SEQ ID NO:

1699, wherein the isolated nucleic acid encodes a polypeptide that provides protective immunity against an infection by S. pneumoniae.

5. A recombinant expression vector comprising the nucleic acid of claim 4 operably linked to a transcription regulatory element.

6. A cell comprising the recombinant expression vector of claim 5.

7. An isolated nucleic acid selected from the group consisting of:

a) a nucleic acid having at least 99% identity to SEQ ID NO;

1699;

b) a nucleic acid fully complementary to a nucleic acid having at least 99% identity to SEQ ID NO;

1699; and

c) an RNA of a) or b), wherein U is substituted for T;

wherein the isolated nucleic acid encodes a polypeptide that provides protective immunity against an infection by S. pneumoniae.

8. A recombinant expression vector comprising the nucleic acid of claim 7 operably linked to a transcription regulatory element.

9. A cell comprising the recombinant expression vector of claim 8.

10. An isolated nucleic acid selected from the group consisting of:

a) a nucleic acid encoding a S. pneumoniae polypeptide having at least 98% identity to SEQ ID NO;

4360;

b) a nucleic acid fully complementary to a nucleic acid encoding a polypeptide having at least 98% identity to SEQ ID NO;

4360; and

c) an RNA of a) or b), wherein U is substituted for T;

wherein the isolated nucleic acid encodes a polypeptide that provides protective immunity against an infection by S. pneumoniae.

11. A recombinant expression vector comprising the nucleic acid of claim 10 operably linked to a transcription regulatory element.

12. A cell comprising the recombinant expression vector of claim 11.