Universal gene chip for high throughput chemogenomic analysis
First Claim
1. A method for preparing a high-throughput chemogenomic assay reagent set comprising:
- a. deriving a set of non-redundant classifiers, each comprising a plurality of genes, from a chemogenomic dataset, wherein the chemogenomic dataset comprises expression levels for a plurality of gene measured in response to a plurality of compound treatments;
b. ranking each gene in the set of non-redundant classifiers based on its contribution across all of the non-redundant classifiers;
c. selecting the subset of genes ranking in about the 50th percentile or higher; and
d. preparing a plurality of isolated polynucleotides or polypeptides, wherein each polynucleotide or polypeptide is capable of detecting at least one gene of the selected subset.
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Abstract
The invention provides methods for preparing reagent sets based on small subsets of highly informative genes capable of carrying out a broad range of chemogenomic classification tasks. The invention also provides high-throughput diagnostic assays and devices based on these reduced subsets of information rich genes. In addition, the invention provides a general method for selecting a reduced subset of highly responsive variables from a much larger multivariate dataset, and thus, use of these variables to prepare diagnostic measurement devices, or other analytic tools, with little or no loss of performance relative to devices or tools incorporating the full set of variables.
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28 Claims
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1. A method for preparing a high-throughput chemogenomic assay reagent set comprising:
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a. deriving a set of non-redundant classifiers, each comprising a plurality of genes, from a chemogenomic dataset, wherein the chemogenomic dataset comprises expression levels for a plurality of gene measured in response to a plurality of compound treatments;
b. ranking each gene in the set of non-redundant classifiers based on its contribution across all of the non-redundant classifiers;
c. selecting the subset of genes ranking in about the 50th percentile or higher; and
d. preparing a plurality of isolated polynucleotides or polypeptides, wherein each polynucleotide or polypeptide is capable of detecting at least one gene of the selected subset. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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- 20. A reagent set for chemogenomic analysis of a compound treated sample, wherein the set comprises a plurality of polynucleotides or polypeptides, wherein each polynucleotide or polypeptide is capable of detecting at least one member of a subset of less than about 10 percent of the genes in a full chemogenomic dataset, and wherein the subset of genes is capable of generating a set of signatures that exhibit at least about 85 percent of the average performance of the same set of signatures generated from the full chemogenomic dataset.
Specification