Devices and methods for enrichment and alteration of circulating tumor cells and other particles
First Claim
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1. A device for processing a cellular sample, said device comprising:
- a) a channel comprising a structure that directs one or more first cells in a first direction to produce a first output sample enriched in said first cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells, wherein said device is configured either;
i) to direct cells having a hydrodynamic size greater than 12 microns in said first direction, and cells having a hydrodynamic size less than or equal to 12 microns in said second direction;
or ii) to direct cells having a hydrodynamic size greater than or equal to 6 microns and less than or equal to 12 microns in said first direction, and cells having a hydrodynamic size less than 6 microns or cells having a hydrodynamic size greater than 12 microns in said second direction; and
b) a detection module for analyzing said first output sample or said second output sample, wherein said detection module is fluidically coupled to said channel.
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Abstract
The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
179 Citations
30 Claims
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1. A device for processing a cellular sample, said device comprising:
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a) a channel comprising a structure that directs one or more first cells in a first direction to produce a first output sample enriched in said first cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells, wherein said device is configured either;
i) to direct cells having a hydrodynamic size greater than 12 microns in said first direction, and cells having a hydrodynamic size less than or equal to 12 microns in said second direction;
orii) to direct cells having a hydrodynamic size greater than or equal to 6 microns and less than or equal to 12 microns in said first direction, and cells having a hydrodynamic size less than 6 microns or cells having a hydrodynamic size greater than 12 microns in said second direction; and
b) a detection module for analyzing said first output sample or said second output sample, wherein said detection module is fluidically coupled to said channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 30)
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9. A device for processing a cellular sample, said device comprising:
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a) a channel comprising a structure that directs one or more cancer cells in a first direction to produce a first output sample enriched in said cancer cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells; and
b) a capture module for capturing said cancer cells or said second cells, wherein said capture module is fluidically coupled to said channel, and wherein said capture module comprises one or more binding moieties that selectively bind said cancer cells or said second cells. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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- 21. A device for processing a cellular sample, said device comprising a channel comprising a structure that directs one or more first cells in a first direction to produce a first output sample enriched in said first cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells, wherein said structure comprises an array of obstacles that form a network of gaps, and wherein at least some of said obstacles comprise monoclonal anti-EpCAM antibodies or fragments thereof that selectively bind said first cells or said second cells.
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22. A device for processing a cellular sample, said device comprising:
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a) an enrichment module that is capable of enriching cells in said cellular sample based on size; and
b) a cell counting module for determining the number of cells enriched by said enrichment module, wherein said cell counting module is fluidically coupled to said enrichment module. - View Dependent Claims (23, 24, 25, 26, 27, 28)
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Specification