Devices and methods for enrichment and alteration of circulating tumor cells and other particles
First Claim
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1. A method for diagnosing a condition in a subject, said method comprising the steps of:
- a) introducing a cellular sample from said subject into a device comprising a channel comprising a structure that directs one or more first cells in a first direction to produce a first output sample enriched in said first cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells;
b) analyzing said first output sample; and
c) diagnosing the presence or absence of said condition based on the results of step b).
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Abstract
The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
204 Citations
64 Claims
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1. A method for diagnosing a condition in a subject, said method comprising the steps of:
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a) introducing a cellular sample from said subject into a device comprising a channel comprising a structure that directs one or more first cells in a first direction to produce a first output sample enriched in said first cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells;
b) analyzing said first output sample; and
c) diagnosing the presence or absence of said condition based on the results of step b). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 64)
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37. A method for identifying a cell pattern associated with a condition of interest, said method comprising the steps of:
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a) obtaining a cellular sample from each of a plurality of control subjects and a plurality of case subjects having said condition of interest;
b) enriching by size, from each said cellular sample, cells having a hydrodynamic size greater than 12 microns;
c) analyzing cells enriched in step b); and
d) performing an association study using the results obtained in step c). - View Dependent Claims (39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56)
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38. A method for diagnosing a condition in a subject, said method comprising the steps of:
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a) providing a cell pattern associated with said condition;
b) obtaining a cellular sample from said subject;
c) enriching by size, from said cellular sample, cells having a hydrodynamic size greater than 12 microns;
d) analyzing cells enriched in step c); and
e) diagnosing the presence or absence of said condition in said subject based on said cell pattern of step a) together with said analysis of step d).
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57. A method for determining the efficacy of a drug treatment administered to a subject, said method comprising the steps of:
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a) obtaining a first cellular sample from said subject before said treatment;
b) introducing said first cellular sample into a device comprising a channel comprising a structure that directs one or more first cells in a first direction to produce a first output sample enriched in said first cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells;
c) analyzing said first output sample;
d) obtaining a second cellular sample from said subject concurrently with or subsequent to said drug treatment;
e) repeating steps b) and c) for said second cellular sample; and
f) comparing the results of step c) for said first cellular sample and said second cellular sample, wherein said comparing determines said efficacy of said drug treatment. - View Dependent Claims (58, 59, 60, 61, 62, 63)
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Specification