Devices and methods for enrichment and alteration of circulating tumor cells and other particles
First Claim
Patent Images
1. A method of detecting cancer cells in a cellular sample, said method comprising the steps of:
- a) introducing said cellular sample into a device comprising a channel comprising a structure that directs said cancer cells in a first direction to produce a first output sample enriched in said cancer cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells; and
b) detecting the presence or absence of said cancer cells in said first output sample.
5 Assignments
0 Petitions
Accused Products
Abstract
The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
191 Citations
43 Claims
-
1. A method of detecting cancer cells in a cellular sample, said method comprising the steps of:
-
a) introducing said cellular sample into a device comprising a channel comprising a structure that directs said cancer cells in a first direction to produce a first output sample enriched in said cancer cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells; and
b) detecting the presence or absence of said cancer cells in said first output sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 34, 35, 36, 43)
-
-
26. A method of detecting cancer cells in a cellular sample, said method comprising the steps of:
-
a) enriching one or more of said cancer cells from said cellular sample without using magnetic particles, wherein said enriching is based on cell size, shape, or deformability; and
b) determining the number of said enriched cancer cells. - View Dependent Claims (28, 29, 30, 31, 32, 33)
-
-
27. A method of detecting cancer cells in a cellular sample, said method comprising the steps of:
-
a) enriching one or more of said cancer cells from said cellular sample without using an antibody or fragment thereof, wherein said enriching is based on cell size, shape, or deformability; and
b) determining the number of said enriching cancer cells.
-
-
37. A method for diagnosing a condition in a subject, said method comprising the steps of:
-
a) introducing a cellular sample from said subject into a device comprising a channel comprising a structure that directs one or more cancer cells in a first direction to produce a first output sample enriched in said cancer cells and one or more second cells in a second direction to produce a second output sample enriched in said second cells;
b) detecting the presence or absence of said cancer cells in said first output sample; and
c) diagnosing the presence or absence of said condition based on the results of step b). - View Dependent Claims (38, 39, 40, 41, 42)
-
Specification