Devices and methods for enrichment and alteration of circulating tumor cells and other particles
First Claim
1. A two-dimensional array of obstacles that form a network of gaps, wherein said array of obstacles comprises a plurality of rows, each successive row being offset by less than half of the period of the previous row, and wherein at least 70% of gaps between obstacles each has a length approximately equal to a first length parameter and at most 30% of gaps between obstacles each has a length approximately equal to a second length parameter, wherein said first length parameter is larger than said second length parameter.
2 Assignments
0 Petitions
Accused Products
Abstract
The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.
-
Citations
55 Claims
- 1. A two-dimensional array of obstacles that form a network of gaps, wherein said array of obstacles comprises a plurality of rows, each successive row being offset by less than half of the period of the previous row, and wherein at least 70% of gaps between obstacles each has a length approximately equal to a first length parameter and at most 30% of gaps between obstacles each has a length approximately equal to a second length parameter, wherein said first length parameter is larger than said second length parameter.
- 17. A two-dimensional array of obstacles that form a network of gaps, wherein said array of obstacles comprises a plurality of rows, each successive row being offset by less than half of the period of the previous row, and wherein at most 20% of said obstacles'"'"' centers are unaligned with the centers of the remaining obstacles in their row.
- 20. A two-dimensional array of obstacles that form a network of gaps, wherein said array of obstacles comprises a plurality of rows, each successive row being offset by less than half of the period of the previous row, and wherein at least 80% of said obstacles each has a cross-sectional area approximately equal to a first area parameter and at most 20% of said obstacles each has a cross-sectional area approximately equal to a second area parameter.
- 24. A two-dimensional array of obstacles comprising a first subarray of obstacles and a second subarray of obstacles, wherein each of said subarrays of obstacles comprises a first gap between two obstacles in said subarray, and wherein said two-dimensional array comprises an interface between said first subarray and said second subarray, said interface comprising a restricted gap smaller than said first gap.
-
42. A device comprising:
-
a) a two-dimensional array of obstacles that form a network of gaps, wherein said array of obstacles comprises a plurality of rows, each successive row being offset by less than half of the period of the previous row, and wherein at least 70% of gaps between obstacles each has a length approximately equal to a first length parameter and at most 30% of gaps between obstacles each has a length approximately equal to a second length parameter, wherein said first length parameter is longer than said second length parameter;
b) a sample reservoir fluidically coupled to said array; and
c) a detector fluidically coupled to said array. - View Dependent Claims (43)
-
-
44. A method for removing an analyte from a cellular sample, said method comprising processing said cellular sample using a device comprising:
-
a) a two-dimensional array of obstacles that form a network of gaps, wherein said array of obstacles comprises a plurality of rows, each successive row being offset by less than half of the period of the previous row, and wherein at least 70% of gaps between obstacles each has a length approximately equal to a first length parameter and at most 30% of gaps between obstacles each has a length approximately equal to a second length parameter, wherein said first length parameter is longer than said second length parameter;
b) a sample reservoir fluidically coupled to said array; and
c) a detector fluidically coupled to said array. - View Dependent Claims (45, 46, 47, 48, 49)
-
- 50. A device for the enrichment of a first analyte from a fluid sample, said device comprising a two-dimensional array of obstacles that form a network of gaps, wherein said array of obstacles comprises a plurality of rows, said rows distributed on a surface to create fluid flow paths through said device, wherein at least 70% of said flow paths each has a width approximately equal to a first width parameter and at most 30% of said flow paths each has a width approximately equal to a second, smaller width parameter.
Specification