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Method and apparatus for generating thermal melting curves in a microfluidic device

  • US 20070026421A1
  • Filed: 02/09/2006
  • Published: 02/01/2007
  • Est. Priority Date: 11/16/2000
  • Status: Active Grant
First Claim
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1. A method of performing thermal melt analysis of a nucleic acid in a microfluidic device, the method comprising:

  • providing a microfluidic device having at least one microfluidic channel, the at least one microfluidic channel having an upstream portion and a downstream portion, introducing fluid comprising the nucleic acid and amplification reagents into the microfluidic channel so that the fluid flows from the upstream portion to the downstream portion of the channel, cycling the temperature in the upstream portion so that the nucleic acid undergoes amplification, subjecting the fluid to a series of temperatures in the downstream portion, wherein the series of temperatures includes a temperature high enough to cause denaturization of the nucleic acid, and measuring a detectable property emanating from the fluid that is indicative of the extent of denaturation of the nucleic acid in the downstream portion.

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