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Methods of producing and sequencing modified polynucleotides

  • US 20070026438A1
  • Filed: 06/28/2006
  • Published: 02/01/2007
  • Est. Priority Date: 06/28/2005
  • Status: Active Grant
First Claim
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1. A method for producing a modified polynucleotide sequence comprising annealing at least one primer to a template polynucleotide sequence and extending said primer in the presence one or more nucleoside triphosphates, wherein at least one of the nucleoside triphosphates is modified wherein said modified nucleotide sequence comprises a phosphorothiolate linkage, thereby producing a modified nucleotide sequence.

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