Fluid processing device and method
First Claim
1. A fluid processing device, comprising:
- a substrate comprising a first surface and an opposing second surface; and
one or more fluid processing pathways at least partially defined by the substrate, the one or more fluid processing pathways each comprising a first region comprising pre-amplification reaction components disposed therein and adapted to pre-amplify a plurality of different nucleic acid sequences present in a sample to produce a plurality of pre-amplified sequences, and two or more second regions each in fluid communication with the first region and comprising amplification reaction components disposed therein adapted to amplify one or more of the plurality of pre-amplified sequences to produce one or more amplified target sequences.
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Accused Products
Abstract
A fluid processing device and methods are provided that can process one or many different fluid samples, detection for each of which can be multiplexed to detect the presence or absence of each of a panel of target sequences, for example 20 different target sequences. The device can comprise a substrate and one or more fluid processing pathways at least partially defined by the substrate. Each fluid processing pathway can comprise a pre-amplification region and two or more amplification regions disposed downstream from and in fluid communication with the pre-amplification region. A burstable valve can be disposed along each fluid processing pathway and the downstream regions can contain pre-loaded ammonia gas to draw an amplified sample downstream.
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Citations
20 Claims
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1. A fluid processing device, comprising:
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a substrate comprising a first surface and an opposing second surface; and
one or more fluid processing pathways at least partially defined by the substrate, the one or more fluid processing pathways each comprising a first region comprising pre-amplification reaction components disposed therein and adapted to pre-amplify a plurality of different nucleic acid sequences present in a sample to produce a plurality of pre-amplified sequences, and two or more second regions each in fluid communication with the first region and comprising amplification reaction components disposed therein adapted to amplify one or more of the plurality of pre-amplified sequences to produce one or more amplified target sequences. - View Dependent Claims (2, 3, 4)
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5. A fluid processing device, comprising:
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a substrate having a first surface and an opposing second surface; and
one or more fluid processing pathways at least partially defined by the substrate, the one or more fluid processing pathways each comprising at least one heat-mediated, pressure-actuated valve adapted to burst when a pressure of at least two atmospheres is exerted across the valve and the valve is heated to a temperature of from about 100°
C. to about 130°
C.
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6. A fluid processing device comprising:
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a substrate having a first surface and an opposing second surface; and
one or more fluid processing pathways at least partially defined by the substrate, the one or more fluid processing pathways each comprising a first region; and
one or more sealed regions disposed downstream from and in fluid communication with the first region, the one or more sealed regions comprising ammonia gas. - View Dependent Claims (7, 8, 9)
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10. A method, comprising:
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providing a fluid processing device comprising one or more fluid processing pathways, each fluid processing pathway comprising a first region in fluid communication with two or more second regions;
introducing a fluid sample comprising a plurality of different nucleic acid sequences from at least one template, into the first region of the fluid processing device;
pre-amplifying two or more of the plurality of different nucleic acid sequences in the first region to produce a pre-amplified fluid sample comprising a plurality of pre-amplified nucleic acid sequences;
moving the pre-amplified fluid sample from the first region to the two or more second regions; and
amplifying at least one respective target nucleic acid sequence of the plurality of pre-amplified nucleic acid sequences in each of the two or more second regions, to produce an amplified fluid sample comprising at least one respective amplified target nucleic acid sequence in each of the two or more second regions. - View Dependent Claims (11, 12, 13, 14, 15)
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16. A method, comprising:
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providing a fluid processing device comprising one or more fluid processing pathways each comprising a first region, and at least one sealed region disposed downstream from and in fluid communication with the first region, wherein the at least one sealed region comprises ammonia gas;
retaining a fluid sample in the first region;
contacting the ammonia gas contained in the at least one sealed region with the fluid sample, wherein the fluid sample is drawn into the at least one sealed region as the ammonia gas dissolves into the fluid sample. - View Dependent Claims (17, 18, 19, 20)
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Specification