Rice plants having increased tolerance to imidazolinone herbicides
First Claim
1. A rice plant comprising a variant AHAS nucleic acid, wherein the variant AHAS nucleic acid confers upon the plant increased tolerance to an imidazolinone herbicide as compared to a wild-type variety of the plant, wherein the variant AHAS nucleic acid encodes a variant AHAS polypeptide comprising an alanine to threonine substitution as compared to a wild-type AHAS polypeptide and wherein the alanine to threonine substitution corresponds to position 96 of the AHAS amino acid sequence as shown in SEQ ID NO:
- 12.
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Abstract
The present invention is directed to plants having increased tolerance to an imidazolinone herbicide. More particularly, the present invention includes rice plants containing at least one variant AHAS nucleic acid such as an imidazolinone tolerant IMINTA 1, 4 or 5 lines comprising an alanine to threonine substitution as compared to the wild-type AHAS. The present invention also includes seeds produced by these rice plants and methods of controlling weeds in the vicinity of these rice plants.
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Citations
46 Claims
- 1. A rice plant comprising a variant AHAS nucleic acid, wherein the variant AHAS nucleic acid confers upon the plant increased tolerance to an imidazolinone herbicide as compared to a wild-type variety of the plant, wherein the variant AHAS nucleic acid encodes a variant AHAS polypeptide comprising an alanine to threonine substitution as compared to a wild-type AHAS polypeptide and wherein the alanine to threonine substitution corresponds to position 96 of the AHAS amino acid sequence as shown in SEQ ID NO:
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23. A rice plant having the herbicide tolerance characteristics of the plant with a Patent Deposit Designation Number NCIMB 41206, NCIMB 41207, or NCIMB 41208, and wherein the plant is selected from the group consisting of:
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(a) a plant having a Patent Deposit Designation Number NCIMB 41206, NCIMB 41207, or NCIMB 41208;
(b) a plant that is a recombinant or genetically engineered derivative of the plant with Patent Deposit Designation Number NCIMB 41206, NCIMB 41207, or NCIMB 41208;
(c) any progeny of the plant of Patent Deposit Designation Number NCIMB 41206, NCIMB 41207, orNCIMB 41208;
or(d) a plant that is a progeny of any of the plants of the plants of (a) through (c). - View Dependent Claims (24, 25, 26, 27, 28, 29, 30)
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31. An isolated AHAS nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
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(a) a polynucleotide as defined in SEQ ID NO;
1, SEQ ID NO;
3, or SEQ ID NO;
5;
(b) a polynucleotide as defined in SEQ ID NO;
11;
(c) a polynucleotide encoding a polypeptide as defined in SEQ ID NO;
2, SEQ ID NO;
4, or SEQ ID NO;
6;
(d) a polynucleotide encoding a polypeptide as defined in SEQ ID NO;
12;
(e) a polynucleotide comprising at least 60 consecutive nucleotides of any of a) through d) above; and
(f) a polynucleotide complementary to the polynucleotide of any of a) through e) above, wherein the isolated AHAS nucleic acid encodes an AHAS polypeptide conferring increased tolerance to an imidazolinone herbicide as compared to a wild-type AHAS polypeptide. - View Dependent Claims (32, 33, 34, 35, 36, 37)
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38. An isolated AHAS polypeptide, wherein the polypeptide is selected from the group of polypeptide sequences as defined in SEQ ID NO:
- 2, SEQ ID NO;
4, SEQ ID NO;
6, and SEQ ID NO;
12, wherein the polypeptide confers increased tolerance to an imidazolinone herbicide as compared to a wild-type AHAS polypeptide. - View Dependent Claims (39, 40, 41)
- 2, SEQ ID NO;
- 42. A method of controlling weeds within the vicinity of a rice plant, comprising applying an imidazolinone herbicide to the weeds and the plant, wherein the plant has increased tolerance to the imidazolinone herbicide as compared to a wild-type variety of the plant, wherein the plant comprises a variant AHAS nucleic acid encoding a variant AHAS polypeptide comprising an alanine to threonine substitution as compared to a wild-type AHAS polypeptide and wherein the alanine to threonine substitution corresponds to position 96 of the AHAS amino acid sequence as shown in SEQ ID NO:
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44. A method of producing a transgenic plant having increased tolerance to an imidazolinone herbicide comprising,
(a) transforming a plant cell with one or more expression vectors comprising at least one variant AHAS nucleic acid encoding a variant AHAS polypeptide comprising an alanine to threonine substitution as compared to a wild-type AHAS polypeptide, wherein the alanine to threonine substitution corresponds to position 96 of the AHAS amino acid sequence as shown in SEQ ID NO: - 12.; and
(b) generating from the plant cell a transgenic plant with an increased tolerance to an imidazolinone herbicide as compared to a wild-type variety of the plant. - View Dependent Claims (45)
- 12.; and
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46. A method of controlling weeds within the vicinity of a rice plant, comprising applying an imidazolinone herbicide to the weeds and the plant, wherein the plant comprises the herbicide tolerance characteristics of the plant with a Patent Deposit Designation Number NCIMB 41206, NCIMB 41207, or NCIMB 41208, and wherein the plant is selected from the group consisting of:
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(a) a plant having a Patent Deposit Designation Number NCIMB 41206, NCIMB 41207, or NCIMB 41208;
(b) a plant that is a recombinant or genetically engineered derivative of the plant with Patent Deposit Designation Number NCIMB 41206, NCIMB 41207, or NCIMB 41208;
(c) any progeny of the plant of Patent Deposit Designation Number NCIMB 41206, NCIMB 41207, or NCIMB 41208;
or(d) a plant that is a progeny of any of the plants of the plants of (a) through (c).
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Specification