Multiple laminar flow-based particle and cellular separation with laser steering
First Claim
1. A method of separating blood into components, the method comprising:
- providing a first flow having a plurality of blood components;
providing a second flow;
contacting the first flow with the second flow to provide a first separation region; and
differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow.
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Accused Products
Abstract
The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.
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Citations
71 Claims
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1. A method of separating blood into components, the method comprising:
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providing a first flow having a plurality of blood components;
providing a second flow;
contacting the first flow with the second flow to provide a first separation region; and
differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method of separating a fluid mixture into constituent components, the method comprising:
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providing a substantially laminar first flow having the fluid mixture, the fluid mixture having a plurality of components, the plurality of components having a corresponding plurality of sedimentation rates;
providing a substantially laminar second flow;
contacting the first flow with the second flow to provide a first separation region, the first flow and the second flow having a substantially non-turbulent interface within the separation region;
differentially sedimenting from the first flow a first component of the plurality of components into the second flow to form an enriched second flow and a depleted first flow, while concurrently maintaining a second component of the plurality of components in the first flow, the first component having a first sedimentation rate of the plurality of sedimentation rates and the second component having a second sedimentation rate of the plurality of sedimentation rates, wherein the first sedimentation rate is comparatively greater than the second sedimentation rate;
differentially removing the enriched second flow from the depleted first flow; and
holographically manipulating the second component in the depleted first flow. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29)
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30. A method of separating a fluid mixture into constituent components, the method comprising:
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providing a substantially laminar first flow having the fluid mixture, the fluid mixture having a plurality of components, the plurality of components having a corresponding plurality of sedimentation rates;
providing a substantially laminar second flow;
providing a substantially laminar third flow;
contacting the first flow with the second flow and with the third flow to provide a first separation region, the first flow and the second flow having a substantially non-turbulent first interface within the separation region, and the first flow and the third flow having a substantially non-turbulent second interface within the separation region;
differentially removing from the first flow a first component of the plurality of components into the second flow to form an enriched second flow and a depleted first flow;
differentially removing from the first flow a second component of the plurality of components into the third flow to form an enriched third flow and a further depleted first flow;
differentially removing the enriched second flow from the depleted first flow; and
differentially removing the enriched third flow from the depleted first flow. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38)
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39. An apparatus for separating a fluid mixture into constituent components, the apparatus comprising:
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means for providing a substantially laminar first flow having the fluid mixture, the fluid mixture having a plurality of components, the plurality of components having a corresponding plurality of sedimentation rates;
means for providing a substantially laminar second flow;
means for providing a substantially laminar third flow;
means for contacting the first flow with the second flow and with the third flow to provide a first separation region, the first flow and the second flow having a substantially non-turbulent first interface within the separation region, and the first flow and the third flow having a substantially non-turbulent second interface within the separation region;
means for differentially removing from the first flow a first component of the plurality of components into the second flow to form an enriched second flow and a depleted first flow;
means for differentially removing from the first flow a second component of the plurality of components into the third flow to form an enriched third flow and a further depleted first flow;
means for differentially removing the enriched second flow from the depleted first flow; and
means for differentially removing the enriched third flow from the depleted first flow. - View Dependent Claims (40, 41, 42, 43, 44, 45)
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46. A method of separating blood into components, the method comprising:
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providing a substantially laminar first flow having a plurality of blood components;
providing a substantially laminar second flow;
contacting the first flow with the second flow to provide a first separation region, a size of the first separation region having been predetermined based on a plurality of sedimentation rates of the plurality of blood components;
differentially rate zonal sedimenting a plurality of red blood cells and a plurality of white blood cells, of the plurality of blood components, into the second flow while concurrently maintaining a plurality of platelets and plasma, of the plurality of blood components, in the first flow;
differentially removing the second flow having the first blood cellular component from the first flow having the second blood cellular component;
providing a third flow;
contacting the first flow with the third flow to provide a second separation region;
differentially rate zonal sedimenting the plurality of platelets into the third flow while concurrently maintaining plasma in the first flow; and
recirculating the first flow to form the second flow.
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47. An apparatus for separating a plurality of components in a fluid, the apparatus comprising:
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an optically transparent sorting channel having a first inlet for a first flow and a second inlet for a second flow, the optically transparent sorting channel further having a first outlet for the first flow and a second outlet for the second flow; and
a holographic optical trap system coupled to the optically transparent sorting channel, the holographic optical trap system adapted to generate a holographic optical trap to select and move a first component in the first flow, of a plurality of components in the first flow, into the second flow to form an enriched second flow and a depleted first flow, while a second component of the plurality of components is concurrently maintained in the first flow.
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48. An apparatus for separating a fluid mixture into constituent, non-motile components, the apparatus comprising:
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a first sorting channel having a first inlet for a first flow and a second inlet for a second flow;
the first sorting channel further having a first outlet for the first flow and a second outlet for the second flow, the first sorting channel adapted to allow a first component in the first flow, of a plurality of components in the first flow, to sediment into the second flow to form an enriched second flow and a depleted first flow, while concurrently maintaining a second component of the plurality of components in the first flow;
a second, optically transparent sorting channel having a first optical inlet coupled to the first outlet for the first flow and having a first optical outlet, the second, optically transparent sorting channel further having a second optical inlet for a third flow and a second optical outlet for the third flow; and
a holographic optical trap system coupled to the second, optically transparent sorting channel, the holographic optical trap system adapted to generate a holographic optical trap to select and move the second component from the first flow into the third flow.
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49. A method of separating a plurality of cells, the method comprising:
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providing a first flow having the plurality of cells;
providing a second flow;
contacting the first flow with the second flow to provide a first separation region; and
differentially removing a first cell of the plurality of cells into the second flow while concurrently maintaining a second cell of the plurality of cells in the first flow. - View Dependent Claims (50, 51, 52, 53, 54, 55, 56, 57, 58)
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59. An apparatus for separating a plurality of cells, the method comprising:
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means for providing a first flow having the plurality of cells;
means for providing a second flow;
means for contacting the first flow with the second flow to provide a first separation region; and
means for differentially removing a first cell of the plurality of cells into the second flow while concurrently maintaining a second cell of the plurality of cells in the first flow. - View Dependent Claims (60, 61, 62, 63, 64, 65, 66, 67)
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68. An apparatus for separating a component from a plurality of components in a fluid, the apparatus having an aspect ratio of length to width, the apparatus comprising:
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a first sorting channel having a first inlet for a first flow and a second inlet for a second flow;
the first and second flows each having a flow direction along the length of the apparatus, the first sorting channel further having a first outlet for the first flow and a second outlet for the second flow, the first sorting channel adapted to allow a first component in the first flow, of a plurality of components in the first flow, to be selectively removed into the second flow to form an enriched second flow and a depleted first flow, while concurrently maintaining a second component of the plurality of components in the first flow; and
wherein the aspect ratio of length to width is less than about 2;
1. - View Dependent Claims (69, 70)
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71. (canceled)
Specification