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Isolation of CpG islands by thermal segregation and enzymatic selection-amplification method

  • US 20070031858A1
  • Filed: 03/02/2006
  • Published: 02/08/2007
  • Est. Priority Date: 08/02/2005
  • Status: Active Grant
First Claim
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1. A method of amplifying a plurality of amplifiable DNA molecules, comprising:

  • providing a plurality of DNA molecules, said plurality having molecules comprising one or more regions that are GC-poor and having molecules comprising one or more regions that are GC-rich;

    subjecting the plurality of DNA molecules to sufficient conditions to denature GC-poor regions but not to denature GC-rich regions, thereby producing GC-rich regions suitable for amplification; and

    subjecting the plurality to amplification conditions such that the denatured GC-poor regions are substantially not amplified and such that one or more of the non-denatured or partially denatured GC-rich regions are amplified.

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