Integration of alternative feedstreams for biomass treatment and utilization
First Claim
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1. A method for treating biomass composed of integrated feedstocks, said method comprising:
- a) providing biomass;
b) adding to the biomass of a) at least one alternative feedstream to produce an integrated feedstock;
c) contacting the integrated feedstock of b) with an aqueous solution comprising ammonia to form an integrated feedstock-aqueous ammonia mixture, wherein the ammonia is present at a concentration at least sufficient to maintain alkaline pH of the integrated feedstock-aqueous ammonia mixture but wherein said ammonia is present at less than about 12 weight percent relative to dry weight of integrated feedstock, and further wherein the dry weight of integrated feedstock is at a high solids concentration of at least about 15 weight percent relative to the weight of the integrated feedstock-aqueous ammonia mixture, to produce a pretreated integrated feedstock product; and
d) contacting the product of c) with a saccharification enzyme consortium under suitable conditions to produce a fermentable sugar product.
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Abstract
The present invention provides a method for treating biomass composed of integrated feedstocks to produce fermentable sugars. One aspect of the methods described herein includes a pretreatment step wherein biomass is integrated with an alternative feedstream and the resulting integrated feedstock, at relatively high concentrations, is treated with a low concentration of ammonia relative to the dry weight of biomass. In another aspect, a high solids concentration of pretreated biomass is integrated with an alternative feedstream for saccharifiaction.
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Citations
39 Claims
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1. A method for treating biomass composed of integrated feedstocks, said method comprising:
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a) providing biomass;
b) adding to the biomass of a) at least one alternative feedstream to produce an integrated feedstock;
c) contacting the integrated feedstock of b) with an aqueous solution comprising ammonia to form an integrated feedstock-aqueous ammonia mixture, wherein the ammonia is present at a concentration at least sufficient to maintain alkaline pH of the integrated feedstock-aqueous ammonia mixture but wherein said ammonia is present at less than about 12 weight percent relative to dry weight of integrated feedstock, and further wherein the dry weight of integrated feedstock is at a high solids concentration of at least about 15 weight percent relative to the weight of the integrated feedstock-aqueous ammonia mixture, to produce a pretreated integrated feedstock product; and
d) contacting the product of c) with a saccharification enzyme consortium under suitable conditions to produce a fermentable sugar product. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 39)
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11. A method of treating biomass composed of integrated feedstocks, said method comprising:
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a) providing biomass;
b) subjecting the biomass of a),to a pretreatment process to produce a pretreated biomass product;
c) adding to the pretreated biomass product of b) at least one alternative feedstream to produce a first or second integrated feedstock; and
d) contacting the first or second integrated feedstock of c) with a saccharification enzyme consortium under suitable conditions, to produce a fermentable sugar product. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 37, 38)
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Specification