System and method for monitoring of end organ oxygenation by measurement of in vivo cellular energy status
First Claim
1. A method of measuring in vivo of an endogenous fluorophore in a tissue site, comprising:
- selecting a known excitation wavelength of the endogenous flurophore within a range of wavelengths at which the endogenous flurophore undergoes fluorescence;
irradiating the tissue site with irradiated light having at least the selected excitation wavelength within the range of wavelengths;
detecting a fluorescence emission of the tissue site resulting from the irradiation thereof;
determining a relative or absolute concentration of the endogenous fluorophore by multiplying it by a calibration factor that depends one at least one of, a known excitation and emission property of the endogenous fluorophore, an intensity of the irradiated light, optical properties of an excitation probe, and specific properties of the tissue;
using the relative or absolute concentration of the endogenous fluorphore to estimate at least one of a, in vivo cellular energy production status or state of end-organ tissue oxygenation.
1 Assignment
0 Petitions
Accused Products
Abstract
A method is provided of measuring in vivo of an endogenous fluorophore in a tissue site. A known excitation wavelength of the endogenous flurophore is selected within a range of wavelengths at which the endogenous flurophore undergoes fluorescence. The tissue site is irradiated with irradiated light having at least the selected excitation wavelength within the range of wavelengths. A fluorescence emission of the tissue site resulting from the irradiation thereof is detected. A relative or absolute concentration of the endogenous fluorophore is determined by multiplying it by a calibration factor that depends one at least one of, a known excitation and emission property of the endogenous fluorophore, an intensity of the irradiated light, optical properties of an excitation probe, and specific properties of the tissue. The relative or absolute concentration of the endogenous fluorphore is used to estimate at least one of a, in vivo cellular energy production status or state of end-organ tissue oxygenation.
68 Citations
94 Claims
-
1. A method of measuring in vivo of an endogenous fluorophore in a tissue site, comprising:
-
selecting a known excitation wavelength of the endogenous flurophore within a range of wavelengths at which the endogenous flurophore undergoes fluorescence;
irradiating the tissue site with irradiated light having at least the selected excitation wavelength within the range of wavelengths;
detecting a fluorescence emission of the tissue site resulting from the irradiation thereof;
determining a relative or absolute concentration of the endogenous fluorophore by multiplying it by a calibration factor that depends one at least one of, a known excitation and emission property of the endogenous fluorophore, an intensity of the irradiated light, optical properties of an excitation probe, and specific properties of the tissue;
using the relative or absolute concentration of the endogenous fluorphore to estimate at least one of a, in vivo cellular energy production status or state of end-organ tissue oxygenation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
-
-
32. A method of measuring in vivo of endogenous fluorophores in a tissue site, comprising:
-
Irradiating the tissue site at multiple irradiation wavelengths with excitation wavelengths known to excite one or more selected endogenous fluorophores;
receiving a measured emission wavelength response of an emitted light intensity for one or more of the excitation wavelengths;
forming an equation for each measured emission wavelength where the measured emission wavelength is equal to a sum of the responses from the selected endogenous fluorophores; and
forming a system of equations where there is an equation for each combination of an irradiation wavelength and measured emission wavelength. - View Dependent Claims (33, 34, 35, 36, 37)
-
-
38. A method of monitoring energy production status of in vivo tissue, comprising:
-
measuring emission of first and second groups of endogenous flurophores, the first group having quantity or emitting characteristics that vary with cellular respiration of oxygen, and the second group having quantity or emitting characteristics that vary with energy production status of mammalian cells; and
determining a concentration of one or more of the endogenous fluorophore;
determining a relative or absolute concentration of the endogenous fluorophore by multiplying it by a calibration factor that depends one at least one of, a known excitation and emission property of the endogenous fluorophore, an intensity of the irradiated light, optical properties of an excitation probe, and specific properties of the tissue;
using the relative or absolute concentration of the endogenous fluorophore to estimate at least one of a, in vivo cellular energy production status or state of end-organ tissue oxygenation. - View Dependent Claims (39, 40, 41, 42, 43, 44, 45)
-
-
46. A system for measuring in vivo at least one of endogenous fluorophores s in a tissue site, comprising:
-
a light source that produces an excitation wavelength of the endogenous flurophore within a range of wavelengths at which the endogenous flurophore undergoes fluorescence;
a detector for detecting a fluorescence emission of the tissue site resulting from the irradiation thereof; and
a processor configured to analyze the detected emission to determine the presence of the endogenous flurophore in the tissue site. - View Dependent Claims (47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94)
-
Specification