METHOD FOR DETECTING OR ASSSAYING TARGET MATERIAL, AND ELECTRODE SUBSTRATE, DEVICE, AND KIT USED FOR THE SAME

• US 20070039834A1
• Filed: 05/16/2006
• Published: 02/22/2007
• Est. Priority Date: 05/20/2005
• Status: Abandoned Application

• Current Assignee: Seiko Epson Corporation (Seiko Group)
• Original Assignee: Seiko Epson Corporation (Seiko Group)
• Inventors: Yokokawa, Shinobu, Fukushima, Hitoshi, Takiguchi, Hiroshi
• Application Number:
  US11/383,699
• Publication Number:
  US 20070039834A1
• Time in Patent Office: Days
• US Class Current: 205/777.500
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

A method for detecting or assaying a target material in a sample solution, including: a first process for forming a metal oxide thin film containing a target material model on an electrode substrate; a second process for forming, on the metal oxide thin film, a recess into which the target material is able to engage, by removing the target material model from the metal oxide thin film; a third process for having the sample solution, into which a redox reactive molecule is added, contact the metal oxide thin film in which the recess is formed; and a forth process for electrochemically detecting a transition of electron exchange with the redox reactive molecule in the vicinity of the electrode substrate surface, before and after the third process.

Claims

1. A method for detecting or assaying a target material in a sample solution, comprising:

a) forming a metal oxide thin film containing a target material model on an electrode substrate;

b) forming, on the metal oxide thin film, a recess into which the target material is able to engage, by removing the target material model from the metal oxide thin film;

c) making the sample solution, into which a redox reactive molecule is added, contact the metal oxide thin film in which the recess is formed; and

d) electrochemically detecting a transition of electron exchange with the redox reactive molecule in the vicinity of the electrode substrate surface, before and after the third process.

2. The method according to claim 1, wherein the target material is selected from a group including an organic molecule, a biomolecule, a cell, a microorganism, and a virus.

3. The method according to claim 1, wherein, in the process b), the metal oxide thin film is formed in such a thickness so that only one layer of the target material model is included therein.

4. The method according to claim 1, wherein, in the process b), the metal oxide thin film is formed with a surface sol-gel approach using a metal alkoxide compound.

5. The method according to claim 1, wherein, in the process b), the removal of the target material model is conducted with a processing selected from a group including:

oxygen plasma processing, ozone oxidation processing, elution processing, and firing processing.

6. The method according to claim 1, wherein, the detection in the process d) of the target material model is conducted with a measurement system selected from a group including:

cyclic voltammetry, potentiostatic, galvanostatic, and impedance measurements.

7. The method according to claim 1, wherein, in the process d), in the case where the electron exchange in the vicinity of the substrate surface, with the redox reactive molecule resolved in the sample solution, declines, the determination is made that the target material exists in the sample solution.

8. An electrode substrate for detecting or assaying a target material in a sample solution, comprising:

a thin film formed of metal oxide on the surface of the electrode substrate; and

wherein, on the thin film, a recess to which the target material is able to engage is formed.

9. The electrode substrate according to claim 8, wherein the recess is formed by forming a metal oxide thin film containing a target material model on the surface of the electrode substrate, and removing the target material model from the metal oxide thin film.

10. A device for detecting or assaying a target material in a sample solution, comprising:

the electrode substrate according to claim 8;

a counter electrode that faces the electrode substrate; and

a reference electrode.

11. The device according to claim 10, further comprising a detecting circuit to which the electrode substrate, the counter electrode, and the reference electrode are independently and electrically connected.

13. A kit for detecting or assaying a target material, comprising:

the device according to claim 10;

and

a redox reactive molecule which performs electron exchange in the vicinity of the electrode substrate.

12. A kit for detecting or assaying a target material in a sample solution, comprising:

an electrode substrate;

a material containing a metal oxide compound for forming a thin film on the electrode substrate; and

a redox reactive molecule which performs electron exchange in the vicinity of the electrode substrate.