Methods of preparing nucleic acid for detection
First Claim
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1. A method of preparing nucleic acid from a polysaccharide-containing sample, said method comprising:
- providing one or more glycosidases to the polysaccharide-containing sample, thereby generating a glycosidase-treated sample.
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Abstract
Methods of preparing nucleic acid from polysaccharide-containing samples for detection by providing one or more glycosidases to the sample to degrade polysaccharides are provided. The nucleic acids can further be extracted from the sample. The method is particularly useful for detecting nucleic acid in samples with high starch content.
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Citations
40 Claims
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1. A method of preparing nucleic acid from a polysaccharide-containing sample, said method comprising:
providing one or more glycosidases to the polysaccharide-containing sample, thereby generating a glycosidase-treated sample. - View Dependent Claims (2, 3, 4, 6, 12, 16, 19, 20, 21, 24, 25, 40)
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5. (canceled)
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7-11. -11. (canceled)
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13-15. -15. (canceled)
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17-18. -18. (canceled)
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22-23. -23. (canceled)
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26. (canceled)
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27. A kit for preparing nucleic acid from a sample comprising:
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a) a glycosidase; and
b) instructions for using the kit. - View Dependent Claims (28, 33, 34, 35, 37, 38)
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29-32. -32. (canceled)
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36. (canceled)
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39. (canceled)
Specification