Method and apparatus for detecting estradiol and metabolites thereof using an acoustic device
First Claim
1. A method for detecting estradiol in a sample, the method comprising the steps of:
- a) introducing a plurality of particles coated with a capture agent capable of binding estradiol into a fluid chamber, wherein at least one surface of the fluid chamber comprises an acoustic device having estradiol bound thereto, and b) monitoring signal output by said acoustic device, thereby detecting estradiol in the sample.
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Accused Products
Abstract
Methods for detecting estradiol and metabolites thereof in a sample are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for estradiol, is combined with the sample to form a plurality of estradiol-particle complexes. The system also includes a transport arrangement for transporting the sample and/or particles to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of estradiol-particle complexes that are bound to the sensor surface.
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Citations
36 Claims
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1. A method for detecting estradiol in a sample, the method comprising the steps of:
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a) introducing a plurality of particles coated with a capture agent capable of binding estradiol into a fluid chamber, wherein at least one surface of the fluid chamber comprises an acoustic device having estradiol bound thereto, and b) monitoring signal output by said acoustic device, thereby detecting estradiol in the sample.
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2. The method of claim 1, wherein the acoustic device is a flexural plate wave device.
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3. The method of claim 1, wherein an increased level of estradiol compared to a reference is indicative of the individual having an increased likelihood of developing breast cancer.
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4. The method of claim 1, wherein prior to being introduced into the fluid chamber, said plurality of particles has been exposed to the sample.
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5. The method of claim 1, wherein the sample has been introduced into the fluid chamber prior to introducing the plurality of particles.
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6. The method of claim 1, wherein the particles are magnetic and further comprising creating a magnetic flux in proximity to the acoustic device to attract at least one of the plurality of magnetic particles toward the at least one surface.
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7. The method of claim 6, wherein the magnetic flux is removed prior to step b).
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8. The method of claim 1, wherein the estradiol is present in the sample at a concentration of about 10 pg/ml.
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9. The method of claim 1, wherein the estradiol is selected from the group consisting of free estradiol, sex hormone-binding globulin (SHBG)-bound estradiol, estrone-3-glucuronide, and estradiol-3-glucuronide.
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10. The method of claim 1, wherein the sample is selected from the group consisting of blood, serum, plasma, urine, saliva, and biopsy material.
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11. The method of claim 1, further comprising comparing the signal output of b) to a control signal.
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12. The method of claim 11, wherein the control signal is a standard curve.
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13. The method of claim 11, wherein the control signal is obtained in the absence of sample.
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14. The method of claim 1, wherein the estradiol is indirectly bound to said surface.
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15. The method of claim 14, wherein said surface is coated with a first member of a binding pair, and the estradiol is bound to a second member of the binding pair.
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16. The method of claim 15, wherein the binding pair is selected from the group consisting of biotin/avidin, biotin/streptavidin, and biotin/neutravidin.
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17. The method of claim 1, wherein the capture agent is an antibody.
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18. A method for detecting estradiol in a sample, the method comprising the steps of:
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a) introducing a plurality of particles and a competitor molecule into a fluid chamber, said particles being coated with a first capture agent capable of binding estradiol, wherein at least one surface of the fluid chamber comprises an acoustic device that has been coated with a second capture agent capable of binding to the competitor molecule, and b) monitoring signal output by said acoustic device, thereby detecting estradiol.
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19. The method of claim 18, wherein the acoustic device is a flexural plate wave device.
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20. The method of claim 18, an increased level of estradiol compared to a reference is indicative of the individual having an increased likelihood of developing breast cancer.
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21. The method of claim 18, wherein prior to being introduced into the fluid chamber, said plurality of particles has been exposed to the sample and to the competitor molecule.
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22. The method of claim 21, wherein the competitor molecule comprises estradiol bound to a tag and the second capture agent is capable of binding to the tag.
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23. The method of claim 22, wherein the tag is biotin.
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24. The method of claim 19, wherein the competitor molecule comprises two or more estradiol molecules bound to a carrier, and the second capture agent is capable of binding estradiol.
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25. The method of claim 24, wherein the carrier is a selected from the group consisting of horseradish peroxidase and albumin.
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26. The method of claim 18, wherein the particles are magnetic and further comprising creating a magnetic flux in proximity to the acoustic device to attract at least one of the plurality of magnetic particles toward the at least one surface.
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27. The method of claim 26, wherein the magnetic flux is removed prior to step b).
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28. The method of claim 18, wherein the estradiol is present in the sample at a concentration of about 10 pg/ml.
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29. The method of claim 18, wherein the estradiol is selected from the group consisting of free estradiol, sex hormone-binding globulin (SHBG)-bound estradiol, estrone-3-glucuronide, and estradiol-3-glucuronide.
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30. The method of claim 18, wherein the sample is selected from the group consisting of blood, serum, plasma, urine, saliva, and biopsy material.
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31. The method of claim 18, further comprising comparing the signal output of b) to a control signal.
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32. The method of claim 31, wherein the control signal is generated in the absence of sample.
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33. The method of claim 31, wherein the control signal is a standard curve.
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34. The method of claim 18, wherein the capture agent is indirectly bound to the at least one surface of the fluid chamber.
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35. The method of claim 34, wherein the at least one surface is coated with a first member of a binding pair, and the capture agent is bound to a second member of the binding pair.
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36. The method of claim 35, wherein the binding pair is selected from the group consisting of biotin/avidin, biotin/streptavidin, and biotin/neutravidin.
Specification