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Amplification of cell populations from embryonic stem cells

  • US 20070042491A1
  • Filed: 08/17/2006
  • Published: 02/22/2007
  • Est. Priority Date: 08/18/2005
  • Status: Abandoned Application
First Claim
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1. A method of culturing embryonic stem cells, comprising:

  • providing a plurality of embryonic stem cells (ESC);

    seeding the ESC on a substrate or suspending them in a culture medium as a single cell culture; and

    incubating the seeded or suspended ESC under conditions in which they can differentiate to obtain a population of cells, wherein the ESC are not stimulated to form embryoid bodies, and wherein the proportion of a predetermined differentiated cell type in the population of cells is at least 2 times greater than the proportion of the predetermined differentiated cell type in a population obtained in the same manner except that the ESC are stimulated to form embryoid bodies.

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