Nitrite detection technique

• US 20070048182A1
• Filed: 08/31/2005
• Published: 03/01/2007
• Est. Priority Date: 08/31/2005
• Status: Active Grant

• Current Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Original Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Inventors: Song, Xuedong, Kaylor, Rosann
• Granted Patent: US 8,003,399 B2
• Time in Patent Office: Days
• US Class Current: 422/61
• CPC Class Codes: G01N 2800/348 Urinary tract infections
• CPC Class Codes: G01N 33/526 the element being adapted for a specific analyte
• CPC Class Codes: Y10T 436/173076 Nitrite or nitrate

Abstract

A diagnostic test kit for detecting the presence or absence of nitrites within a test sample is provided. The test kit comprises an aromatic primary amine that is capable of reacting with a nitrite to form a diazonium ion. The test kit also comprises a lateral flow device that comprises a chromatographic medium and an absorbent material that receives the test sample after flowing through the chromatographic medium. The chromatographic medium defines a detection zone within which is contained a detection reagent (e.g., nucleophilic aromatic amine) that is capable of reacting with the diazonium ion to form an indicator (e.g., azo compound). The indicator exhibits a color that is different than the color of the detection reagent.

Claims

1. A diagnostic test kit for detecting the presence or absence of nitrites within a test sample, the test kit comprising:

an aromatic primary amine that is capable of reacting with a nitrite to form a diazonium ion; and

a lateral flow device that comprises;

a chromatographic medium defining a detection zone within which is contained a detection reagent, the detection reagent being capable of reacting with the diazonium ion to form an indicator, the indicator exhibiting a color that is different than the color of the detection reagent; and

an absorbent material that receives the test sample after flowing through the chromatographic medium.

2. The diagnostic test kit of claim 1, wherein the aromatic primary amine comprises aniline;

4-chloroaniline;

4-bromoaniline;

2,4,6-tribromoaniline;

2,4,6-trichloroaniline;

α

-trifluoro-m-toluidene;

ortho-toluidine;

m- and p-aminophenol;

ortho-tolidine;

sulfanilamide, p-aminobenzoic acid;

1-amino-8-hydroxynaphthalene-3,6-disulphonic acid;

aminoacetoanilide;

aminophenyl ether, p-arsalinic acid;

4-amino-1-naphthalenecarbonitrile, or derivatives thereof.

3. The diagnostic test kit of claim 1, wherein the aromatic primary amine is p-arsalinic acid, sulfanilamide, or a derivative thereof.

4. The diagnostic test kit of claim 1, wherein the detection reagent comprises a nucleophilic aromatic amine.

5. The diagnostic test kit of claim 4, wherein the detection reagent comprises 8-hydroxyjulolidine, N,N-dimethylaniline;

methylenedianiline;

benzidine;

benzoquinoline;

aminoquinoline;

m-phenylenediamine;

α

-trifluoro-m-toluidene;

ortho-toluidine;

m-aminophenol;

ortho-tolidine;

N-1-naphthylethylene diamine, or a derivative thereof.

6. The diagnostic test kit of claim 1, wherein the detection reagent comprises N-1-naphthylethylene diamine or a derivative thereof.

7. The diagnostic test kit of claim 1, wherein the detection reagent comprises a macromolecular moiety and a reactive moiety.

8. The diagnostic test kit of claim 7, wherein the macromolecular moiety is formed from a polymer.

9. The diagnostic test kit of claim 8, wherein the polymer is a polysaccharide.

10. The diagnostic test kit of claim 7, wherein the macromolecular moiety is formed from a particle.

11. The diagnostic test kit of claim 10, wherein the particle is a carboxylated latex particle.

12. The diagnostic test kit of claim 7, wherein the reactive moiety comprises an aromatic amine.

13. The diagnostic test kit of claim 12, wherein the reactive moiety is formed from N-1-naphthylethylene diamine or a derivative thereof.

14. The diagnostic test kit of claim 1, wherein the indicator is an aromatic azo compound.

15. The diagnostic test kit of claim 1, wherein the chromatographic medium is a porous membrane.

16. The diagnostic test kit of claim 1, wherein the aromatic primary amine is disposed on the lateral flow assay device.

17. The diagnostic test kit of claim 16, wherein the lateral flow assay device further comprises a reagent zone within which is contained the aromatic primary amine.

18. The diagnostic test kit of claim 17, further comprising a sample application zone that is located upstream from the reagent zone.

19. The diagnostic test kit of claim 1, wherein the chromatographic medium further comprises a quenching zone located upstream from the detection zone.

20. The diagnostic test kit of claim 19, wherein the quenching zone contains an agent capable of reacting with a contaminant in the test sample.

21. The diagnostic test kit of claim 1, wherein the lateral flow device comprises a control zone that is capable of signaling the presence of the test sample.

22. The diagnostic test kit of claim 21, further comprising a control reagent that is capable being detected within the control zone.

23. The diagnostic test kit of claim 1, wherein the test sample is urine.

24. A diagnostic test kit for detecting the presence or absence of nitrites within urine, the test kit comprising:

an aromatic primary amine that is capable of reacting with a nitrite to form a diazonium ion; and

a lateral flow device that comprises;

a porous membrane defining a detection zone within which is contained a nucleophilic aromatic amine detection reagent, the detection reagent being capable of reacting with the diazonium ion to form an azo indicator, the azo indicator exhibiting a color that is different than the color of the detection reagent; and

an absorbent material that receives the urine after flowing through the porous membrane.

25. The diagnostic test kit of claim 24, wherein the aromatic primary amine is p-arsalinic acid, sulfanilamide, or a derivative thereof.

26. The diagnostic test kit of claim 24, wherein the detection reagent comprises N-1-naphthylethylene diamine or a derivative thereof.

27. The diagnostic test kit of claim 24, wherein the detection reagent comprises a macromolecular moiety and an aromatic amine moiety.

28. The diagnostic test kit of claim 27, wherein the macromolecular moiety is formed from a polymer.

29. The diagnostic test kit of claim 28, wherein the polymer is a polysaccharide.

30. The diagnostic test kit of claim 27, wherein the macromolecular moiety is formed from a particle.

31. The diagnostic test kit of claim 27, wherein the aromatic amine moiety is formed from N-1-naphthylethylene diamine or a derivative thereof.

32. The diagnostic test kit of claim 24, wherein the aromatic primary amine is disposed on the lateral flow assay device.

33. The diagnostic test kit of claim 32, wherein the lateral flow assay device further comprises a reagent zone within which is contained the aromatic primary amine.

34. The diagnostic test kit of claim 33, further comprising a sample application zone that is located upstream from the reagent zone.

35. The diagnostic test kit of claim 24, wherein the chromatographic medium further comprises a quenching zone located upstream from the detection zone.

36. The diagnostic test kit of claim 24, wherein the lateral flow device comprises a control zone that is capable of signaling the presence of the urine.