CYTOKINE RECEPTOR ZCYTOR17 MULTIMERS
First Claim
1. An isolated polynucleotide that encodes a cytokine receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 33-662 of SEQ ID NO:
- 5, wherein the cytokine receptor polypeptide forms a multimeric or heterodimeric cytokine receptor with a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO;
7, and wherein the multimeric or heterodimeric cytokine receptor binds a ligand comprising amino acid residues 27-164 of SEQ ID NO;
2.
0 Assignments
0 Petitions
Accused Products
Abstract
Novel polypeptide combinations, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zcytor17-containing multimeric or heterodimer cytokine receptors that may be used as novel cytokine antagonists, and within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. The present invention also includes methods for producing the multimeric or heterodimeric cytokine receptor, uses therefor and antibodies thereto.
-
Citations
27 Claims
-
1. An isolated polynucleotide that encodes a cytokine receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 33-662 of SEQ ID NO:
- 5, wherein the cytokine receptor polypeptide forms a multimeric or heterodimeric cytokine receptor with a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO;
7, and wherein the multimeric or heterodimeric cytokine receptor binds a ligand comprising amino acid residues 27-164 of SEQ ID NO;
2. - View Dependent Claims (2, 3)
- 5, wherein the cytokine receptor polypeptide forms a multimeric or heterodimeric cytokine receptor with a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO;
-
4. An expression vector comprising the following operably linked elements:
-
a transcription promoter;
a DNA segment encoding a cytokine receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 33-662 of SEQ ID NO;
5; and
a transcription terminator;
wherein the cytokine receptor polypeptide forms a multimeric or heterodimeric cytokine receptor with a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO;
7, and wherein the multimeric or heterodimeric cytokine receptor binds a ligand comprising amino acid residues 27-164 of SEQ ID NO;
2. - View Dependent Claims (5, 6, 10)
-
-
7. An expression vector comprising the following operably linked elements:
-
a) a first transcription promoter;
a first DNA segment encoding a first cytokine receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 33-662 of SEQ ID NO;
5; and
a first transcription terminator; and
b) a second transcription promoter;
a second DNA segment encoding a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO;
7; and
a second transcription terminator;
wherein the first cytokine receptor polypeptide and the second cytokine receptor polypeptide form a multimeric or heterodimeric cytokine receptor; and
wherein the multimeric or heterodimeric cytokine receptor binds to a ligand comprising amino acid residues 27-164 of SEQ ID NO;
2. - View Dependent Claims (8, 9, 11, 14)
-
-
12. A cultured cell comprising:
-
a first expression vector comprising;
a) a first transcription promoter;
b) a first DNA segment encoding a first cytokine receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 33-662 of SEQ ID NO;
5; and
c) a first transcription terminator; and
a second expression vector comprising;
a) a second transcription promoter;
b) a second DNA segment encoding a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO;
7; and
c) a second transcription terminator;
wherein the first cytokine receptor polypeptide and the second cytokine receptor polypeptide form a multimeric or heterodimeric cytokine receptor; and
wherein the multimeric or heterodimeric cytokine receptor binds to a ligand comprising amino acid residues 27-164 of SEQ ID NO;
2. - View Dependent Claims (15)
-
-
13. A method of producing an antibody to a soluble multimeric or heterodimeric cytokine receptor comprising a first cytokine receptor polypeptide consisting of an amino acid sequence having at least 95% sequence identity with amino acid residue 33 to amino acid residue 532 of SEQ ID NO:
- 5 and a second cytokine receptor polypeptide consisting of amino acid residues 28-739 of SEQ ID NO;
7, the method comprising;
inoculating an animal with the soluble multimeric or heterodimeric cytokine receptor, wherein the soluble multimeric or heterodimeric cytokine receptor elicits an immune response in the animal to produce an antibody that specifically binds the soluble multimeric or heterodimeric cytokine receptor; and
isolating the antibody from the animal.
- 5 and a second cytokine receptor polypeptide consisting of amino acid residues 28-739 of SEQ ID NO;
-
16. An isolated polynucleotide that encodes a soluble cytokine receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 33-532 of SEQ ID NO:
- 5 wherein the soluble cytokine receptor polypeptide forms a soluble multimeric or heterodimeric cytokine receptor with a second soluble cytokine receptor comprising amino acid residues 28-739 of SEQ ID NO;
7, and wherein the soluble multimeric or heterodimeric cytokine receptor binds a ligand comprising amino acid residues 27-164 of SEQ ID NO;
2. - View Dependent Claims (17, 18, 19, 20)
- 5 wherein the soluble cytokine receptor polypeptide forms a soluble multimeric or heterodimeric cytokine receptor with a second soluble cytokine receptor comprising amino acid residues 28-739 of SEQ ID NO;
-
21. An expression vector comprising the following operably linked elements:
-
a transcription promoter;
a DNA segment encoding a soluble cytokine receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 33-532 of SEQ ID NO;
5; and
a transcription terminator;
wherein the cytokine receptor polypeptide forms a soluble multimeric or heterodimeric cytokine receptor with a second soluble cytokine receptor polypeptide comprising amino acid residues 28-739 of SEQ ID NO;
7, and wherein the soluble multimeric or heterodimeric cytokine receptor binds a ligand comprising amino acid residues 27-164 of SEQ ID NO;
2. - View Dependent Claims (22, 23, 24)
-
-
25. A composition comprising:
-
an effective amount of a soluble multimeric or heterodimeric cytokine receptor comprising a first soluble cytokine receptor polypeptide comprising an amino acid sequence having at least 95% sequence identity with amino acid residues 33-532 of SEQ ID NO;
5 and a second soluble cytokine receptor comprising amino acid residues 28-739 of SEQ ID NO;
7; and
a pharmaceutically acceptable vehicle. - View Dependent Claims (26, 27)
-
Specification