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Electrochemical affinity biosensor system and methods

  • US 20070054317A1
  • Filed: 07/01/2004
  • Published: 03/08/2007
  • Est. Priority Date: 07/01/2003
  • Status: Active Grant
First Claim
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1. A compound of the formula II:

  • embedded image wherein R and R1 are the same or different and each can be selected from;

    2,2′

    -bipyridyl, 4,4′

    -disubstituted-2,2′

    -bipyridyl, 5-5′

    -disubstituted-2,2′

    -bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, 5,6-disubstituted-1,10-phenanthrolinyl, or N, N′

    -dimethyl 2,2′

    -biimidazole, wherein each substituent is a methyl, ethyl, or phenyl group, and where the R and R1 groups are coordinated to osmium through their nitrogen atoms;

    R2 is hydrogen, methyl, or ethyl;

    -L- is a linker;

    E is a trivalent linker;

    B is a group comprising a ligand capable of binding to a specific analyte binding partner;

    Z is chloro or bromo;

    X is a counter ion; and

    y is selected to provide a neutral salt; and

    m is 2 to 4.

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