LIGHT-ACTIVATED CATION CHANNEL AND USES THEREOF
First Claim
1. A method of predicting potential ion channel modulating properties of a drug comprising:
- (a) expressing a light-activated cation channel protein and an ion channel of interest in a cell;
(b) exposing said cell to light and monitoring a first response in said cell;
(c) exposing said cell to a candidate drug;
(d) further exposing said cell to light and monitoring a second response in said cell;
(e) determining an ion channel modulating property of said candidate drug based on a comparison of said first and second response.
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Abstract
The present invention provides compositions and methods for light-activated cation channel proteins and their uses within cell membranes and subcellular regions. The invention provides for proteins, nucleic acids, vectors and methods for genetically targeted expression of light-activated cation channels to specific cells or defined cell populations. In particular the invention provides millisecond-timescale temporal control of cation channels using moderate light intensities in cells, cell lines, transgenic animals, and humans. The invention provides for optically generating electrical spikes in nerve cells and other excitable cells useful for driving neuronal networks, drug screening, and therapy.
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Citations
9 Claims
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1. A method of predicting potential ion channel modulating properties of a drug comprising:
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(a) expressing a light-activated cation channel protein and an ion channel of interest in a cell;
(b) exposing said cell to light and monitoring a first response in said cell;
(c) exposing said cell to a candidate drug;
(d) further exposing said cell to light and monitoring a second response in said cell;
(e) determining an ion channel modulating property of said candidate drug based on a comparison of said first and second response. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification