Substrate and method of immobilizing protein
First Claim
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1. A substrate having amino groups on a surface thereof, said substrate being prepared by treating a carrier with an amino group-containing silicon compound represented by the following general formula:
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(RO)3Si—
(CH2)k—
(C6H4)1—
(CH2)m—
(NHCH2CH2)n—
NH2 to introduce the amino groups onto a surface of the carrier, wherein R is an alkyl group, k is an integer ≧
1, l is an integer ≧
0, m is an integer ≧
0, and n is an integer ≧
1.
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Abstract
A substrate for the immobilization of proteins is made by treating a carrier with an amino group-containing silicon compound represented by the following general formula:
(RO)3Si—(CH2)k—(C6H4)1—(CH2)m—(NHCH2CH2)n—NH2 (wherein R is an alkyl group, and k is 1, 2, 3 . . . , l is 0, 1, 2, 3 . . . , m is 0, 1, 2, 3 . . . , and n is 1, 2, 3 . . . ).
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Citations
10 Claims
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1. A substrate having amino groups on a surface thereof, said substrate being prepared by treating a carrier with an amino group-containing silicon compound represented by the following general formula:
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(RO)3Si—
(CH2)k—
(C6H4)1—
(CH2)m—
(NHCH2CH2)n—
NH2to introduce the amino groups onto a surface of the carrier, wherein R is an alkyl group, k is an integer ≧
1, l is an integer ≧
0, m is an integer ≧
0, and n is an integer ≧
1.- View Dependent Claims (2, 3)
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4. A method of immobilizing a protein on a substrate having amino groups on a surface thereof, the method comprising contacting the substrate with a buffer solution comprising the protein, wherein the substrate is made by treating a carrier with an amino group-containing silicon compound represented by the following general formula:
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(RO)3Si—
(CH2)k—
(C6H4)1—
(CH2)m—
(NHCH2CH2)n—
NH2to introduce amino groups onto a surface of the carrier, wherein R is an alkyl group, k is an integer ≧
1, l is an integer ≧
0, m is an integer ≧
0, and n is an integer ≧
1.- View Dependent Claims (5, 6, 7, 8, 9, 10)
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Specification